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Fibrinogen and mucin binding activity of EF0737, a novel protein of Enterococcus faecalis

Samira Sabzi1, Rahil Mashhadi2, Mohammad Reza Pourmand1

  • 1Department of Pathobiology, School of Public Health and Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran.

Iranian Journal of Microbiology
|March 1, 2018
PubMed
Abstract

Insights

A novel cell wall protein, EF0737, from Enterococcus faecalis was cloned and expressed. This protein interacts with fibrinogen, mucin, and patient sera, suggesting its role in E. faecalis infections.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Immunology

Background:

  • Enterococcus faecalis is a leading cause of human infections.
  • Pathogenic E. faecalis utilizes surface components for adhesion and immune response induction.
  • EF0737 is a novel cell wall associated protein potentially involved in E. faecalis pathogenesis.

Purpose of the Study:

  • To clone and express the EF0737 protein.
  • To investigate the interaction of EF0737 with plasma proteins and patient sera.

Main Methods:

  • Cloning of the ef0737 gene into expression vectors.
  • Expression and purification of recombinant EF0737 in E. coli.
  • Western blot analysis to detect protein interactions.

Main Results:

  • Successful cloning and expression of EF0737 in E. coli.
  • EF0737 demonstrated binding to fibrinogen and mucin.
  • EF0737 reacted with sera from patients with E. faecalis infections.

Conclusions:

  • EF0737's interactions suggest a role in E. faecalis pathogenesis.
  • Further characterization of EF0737 is needed to understand host-pathogen interactions.
Keywords:
EF0737Enterococcus faecalisFibrinogenMucinPathogenesis

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