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Transplantation of schistosome sporocysts between host snails: A video guide
Gabriel Mouahid1, Anne Rognon1, Ronaldo de Carvalho Augusto1
1Laboratoire Interactions Hôtes-Pathogènes-Environnements (IHPE), UMR 5244 CNRS/UPVD/IFREMER/UM, University of Perpignan Via Domitia, 58 Avenue Paul Alduy, Bât R, F-66860 Perpignan Cedex, France.
Abstract:
Schistosomiasis is an important parasitic disease, touching roughly 200 million people worldwide. The causative agents are different Schistosoma species. Schistosomes have a complex life cycle, with a freshwater snail as intermediate host. After infection, sporocysts develop inside the snail host and give rise to human dwelling larvae. We present here a detailed step-by-step video instruction in English, French, Spanish and Portuguese that shows how these sporocysts can be manipulated and transferred from one snail to another. This procedure provides a technical basis for different types of ex vivo modifications, such as those used in functional genomics studies.
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