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Updated: Feb 13, 2026

Extraction of Structural Extracellular Polymeric Substances from Aerobic Granular Sludge
Published on: September 26, 2016
The model binary/ternary mixtures for actual EPS solution extracted from the activated sludge in MBR using dead-end
Shuang Zhao1, Longyue Shi2, Yu Ma3
1Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Chemical Engineering, Beijing University of Technology, Beijing 100124, China
Abstract:
In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA). Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated. The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%). Meanwhile, when the composition of ternary mixture was SA/BSA/HA = 285.1/150.1/10.2 mg·L-1, the deviation for CFV, αc and R was 7.65%, 19.6% and 7.27%, respectively, while the corresponding values for the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1) were -12%, 1% and 164% respectively. This indicated that the ternary solution demonstrated a more accurate estimation than the binary solution for imitating the filterability of actual EPS solution. Therefore, the ternary mixture could be employed efficiently to replace the actual EPS solution in terms of three target parameters in practice applications.
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