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Related Concept Videos

Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

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Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
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Embryonic Stem Cells00:58

Embryonic Stem Cells

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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Embryonic Stem Cells00:57

Embryonic Stem Cells

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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
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Adult Stem Cells01:33

Adult Stem Cells

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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Distinctive Features of Adult Stem Cells vs Cancer Stem Cells01:18

Distinctive Features of Adult Stem Cells vs Cancer Stem Cells

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A stem cell is an unspecialized cell that can divide without limit as needed and can, under specific conditions, differentiate into specialized cells.
Adult stem cells
Adult stem cells are tissue-specific; hence, they divide to develop the tissue from which they originate. One type of adult stem cell is the epithelial stem cell, which gives rise to the keratinocytes in the multiple layers of epithelial cells in the epidermis of the skin. Adult bone marrow has three distinct types of stem cells:...
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Related Experiment Video

Updated: Feb 13, 2026

Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach
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Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach

Published on: January 8, 2017

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Stem cell conditioned medium for cell-free therapies.

I Vackovcá, S Kubinová

    Ceskoslovenska Fysiologie
    |March 1, 2018
    PubMed
    Summary

    Stem cell therapy is evolving beyond direct cell integration. Stem cell-derived conditioned medium (CM) offers a cell-free therapeutic approach with comparable efficacy and reduced risks for regenerative medicine.

    Area of Science:

    • Regenerative Medicine
    • Cell Biology
    • Biotechnology

    Background:

    • Stem cells are recognized for treating diseases and defects.
    • Their therapeutic impact is increasingly attributed to paracrine activity, secreting regenerative and anti-inflammatory factors.
    • Conditioned medium (CM) from stem cells encapsulates these beneficial secretory products.

    Purpose of the Study:

    • To review the use of stem cell-derived CM in regenerative medicine.
    • To summarize findings from studies utilizing CM from various stem cell types.
    • To explore factors influencing stem cell secretion and CM composition.

    Main Methods:

    • Literature review of preclinical and clinical studies on stem cell-derived CM.
    • Analysis of factors affecting stem cell secretory profiles.

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    CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications
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    Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach
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  • Comparative assessment of cell-based vs. cell-free (CM) therapy.

    • Main Results:

    • Preclinical data indicate CM's therapeutic effects are comparable to direct stem cell application.
    • CM offers advantages such as lower risks, allogeneic administration potential, and mass production feasibility.
    • Various factors can modulate stem cell secretion, impacting CM composition and efficacy.

    Conclusions:

    • Cell-free therapy using stem cell-derived CM is a promising alternative to cell-based therapy.
    • Understanding factors that modify CM composition is crucial for optimizing its therapeutic potential.
    • CM holds significant promise for advancing regenerative medicine applications.