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Updated: Feb 13, 2026

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Published on: September 6, 2013
Enhancing Analytical Separations Using Super-Resolution Microscopy
Nicholas A Moringo1, Hao Shen1, Logan D C Bishop1
1Department of Chemistry, Rice University, Houston, Texas 77251, USA;
Abstract:
Super-resolution microscopy is becoming an invaluable tool to investigate structure and dynamics driving protein interactions at interfaces. In this review, we highlight the applications of super-resolution microscopy for quantifying the physics and chemistry that occur between target proteins and stationary-phase supports during chromatographic separations. Our discussion concentrates on the newfound ability of super-resolved single-protein spectroscopy to inform theoretical parameters via quantification of adsorption-desorption dynamics, protein unfolding, and nanoconfined transport.
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