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Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles
Published on: October 4, 2012
Green Synthesis of Silver Nanoparticles Using Leaf Extract of Common Arrowhead Houseplant and Its Anticandidal
Mohammad Yasir1, Jaspreet Singh1, Manish Kumar Tripathi1
1Department of Biological Science and Engineering, Maulana Azad National Institute of Technology, Bhopal, Madhya Pradesh, India.
Background:
Silver nanoparticles have excellent medical and nonmedical properties and application compared with other metallic nanoparticles. In the present study, fresh leaves of Syngonium podophyllum have been used for synthesis of silver nanoparticles.
Objectives:
In this study, we evaluated the anticandidal activity of S. podophyllum and the synthesized nanoparticles.
Materials And Methods:
In this study, simple and economical procedure was adopted for silver nanoparticles synthesis. S. podophyllum leaf was processed to obtain aqueous extract as a biological material for nanoparticles production. Synthesized nanoparticles were characterized by ultraviolet (UV) spectroscopy, X-ray diffraction (XRD), and atomic force microscopy.
Results:
The progress of silver nanoparticles biosynthesis from leaf extract of S. podophyllum was observed by UV-visible spectroscopy. The peaks maxima were observed at 455 nm for silver nanoparticles synthesized from the leaf extracts of S. podophyllum. XRD diffractogram showing Bragg peaks of face-centered cubic crystalline elemental silver confirming the formation of silver nanoparticles. The minimal inhibitory concentration values of aqueous extracts of S. podophyllum leaf were estimated by broth dilution method and found that the extracts exhibited antifungal activity against Candida albicans. The antifungal activity was also determined using disk diffusion method by measuring the diameter for zone of inhibition.
Conclusion:
S. podophyllum leaf extract shows strong antifungal activity against C. albicans. S. podophyllum could be applied in the fields of medical and pharmaceuticals for formulation of new drugs.
Summary:
The synthesis, characterization, and antifungal activities of silver nanoparticle from Common arrowhead house plant.The silver nanoparticles were confirmed to be spherical in shape.The antifungal activities of the confirmed their therapeutic potential. Abbreviation used: AgNO3: Silver nitrate, MIC: Minimum inhibitory concentration, MTCC: Microbial type culture collection, SPR: Surface plasmon resonance, UV: Ultraviolet, XRD: X-ray diffraction.
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