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Updated: Feb 13, 2026

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
Published on: December 27, 2010
Rapid Sequencing of Complete env Genes from Primary HIV-1 Samples
Melissa Laird Smith1,2,3,4,5,6,7,8,9,10,11, Ben Murrell1,2,3,4,5,6,7,8,9,10,11, Kemal Eren1,2,3,4,5,6,7,8,9,10,11
1Pacific Biosciences, Menlo Park, CA, USA.
Abstract:
The ability to study rapidly evolving viral populations has been constrained by the read length of next-generation sequencing approaches and the sampling depth of single-genome amplification methods. Here, we develop and characterize a method using Pacific Biosciences' Single Molecule, Real-Time (SMRT®) sequencing technology to sequence multiple, intact full-length human immunodeficiency virus-1 env genes amplified from viral RNA populations circulating in blood, and provide computational tools for analyzing and visualizing these data.
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