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Related Experiment Video

Updated: Feb 13, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

Development of a sheathless CE-ESI-MS interface.

Akiyoshi Hirayama¹, Hiroshi Abe¹, Nozomi Yamaguchi¹

¹Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan.

Electrophoresis

|March 2, 2018

Summary

Researchers developed a novel sheathless interface for capillary electrophoresis-mass spectrometry (CE-MS) to boost sensitivity. This cost-effective interface significantly enhances metabolite detection in complex biological samples.

Keywords:

Cancer cell Capillary electrophoresis Mass spectrometry Metabolomics Sheathless interface

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Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry CE-ICP-MS for Quantification of Iron Redox Species FeII, FeIII

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry CE-ICP-MS for Quantification of Iron Redox Species FeII, FeIII

Published on: May 4, 2020

Area of Science:

Analytical Chemistry
Biochemistry
Mass Spectrometry

Background:

Conventional sheath-flow interfaces in capillary electrophoresis-mass spectrometry (CE-MS) suffer from analyte dilution by sheath liquid, leading to reduced sensitivity.
Improving the sensitivity of CE-MS is crucial for comprehensive metabolomic analysis, especially when dealing with limited sample quantities.

Purpose of the Study:

To develop and validate a novel sheathless interface for CE-MS to overcome the sensitivity limitations of traditional sheath-flow interfaces.
To demonstrate the enhanced performance of the sheathless interface for the analysis of cationic metabolites.

Main Methods:

Fabrication of a sheathless CE-MS interface using a cracked capillary column covered with a dialysis membrane.
Optimization of separation voltage and pressure injection parameters for CE-MS analysis.
Separation and detection of 52 cationic metabolite standards and analysis of cationic metabolites from cancer cells.

Main Results:

The sheathless interface achieved detection limits between 0.06 and 1.7 μmol/L for tested cationic metabolites.
Analysis of cationic metabolites from cancer cells using the sheathless interface yielded approximately 2.5 times more detected peaks compared to conventional sheath-flow CE-MS.
The interface is easy to construct, cost-effective, and adaptable to commercial capillaries.

Conclusions:

The developed sheathless CE-MS interface significantly improves sensitivity and detection capabilities.
This innovative interface offers a powerful and cost-effective tool for highly sensitive metabolomic studies.
The method enables more comprehensive analysis of low-abundance metabolites in biological samples.