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Updated: Feb 13, 2026

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
Published on: August 4, 2009
Kallikrein-related peptidase 14 is the second KLK protease targeted by the serpin vaspin
David Ulbricht1, Catherine A Tindall1, Kathrin Oertwig1
1Institute of Biochemistry, Faculty of Life Sciences, Leipzig University, Brüderstrasse 34, D-04103 Leipzig, Germany.
Abstract:
Kallikrein-related peptidases KLK5, KLK7 and KLK14 are important proteases in skin desquamation and aberrant KLK activity is associated with inflammatory skin diseases such as Netherton syndrome but also with various serious forms of cancer. Previously, we have identified KLK7 as the first protease target of vaspin (Serpin A12). Here, we report KLK14 as a second KLK protease to be inhibited by vaspin. In conclusion, vaspin represents a multi-specific serpin targeting the kallikrein proteases KLK7 and KLK14, with distinct exosites regulating recognition of these target proteases and opposing effects of heparin binding on the inhibition reaction.
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