Development of a two-stage biotransformation system for mercury-contaminated soil remediation

Utilization of bacterial volatilization can be problematic to remediate mercury (Hg)-contaminated soils because most of the Hg in soils is bound to soil particles. The objective of this study was to develop a two-stage system (chemical extraction followed by microbial reduction) for Hg-contaminated soil remediation. The tasks were to (1) select the extraction reagents for Hg extraction, (2) assess the effects of extraction reagents on the growth of Hg-reducing bacterial strains, and (3) evaluate the effectiveness of Ca²⁺ and Mg²⁺ addition on merA gene (Hg reductase) induction. Bacterial inhibition was observed with the addition of 0.1 M ethylenediaminetetraacetic acid or citric acid. Up to 65% of Hg was biotransformed (Hg concentration = 69 mg/kg) from the soils after a 24 h extraction using 0.5 M ammonium thiosulfate. Ca²⁺ and Mg²⁺ were selected because they have the same electric charge as Hg and the studied groundwater contained high concentrations of Ca²⁺ and Mg²⁺. Results showed that the addition of 200 mg/L Ca²⁺ or 650 mg/L Mg²⁺ could reach effective merA induction. In the two-stage experiment, 120 mg/kg Hg-contaminated soils were extracted with 2 rounds of extraction processes for 10 h using 0.5 M ammonium thiosulfate. Approximately 77% of Hg was extracted from the soils after the first-step extraction process. Up to 81% of Hg²⁺ was transformed from the washing solution via the biotransformation processes with Enterobacter cloacae addition and Ca²⁺ and Mg²⁺ supplementation. The two-stage remedial system has the potential to be developed into a practical technology to remediate Hg-contaminated sites.