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MiR-133a/133b inhibits Treg differentiation in IgA nephropathy through targeting FOXP3

Ling-Wei Jin1, Han-Yang Ye1, Xiao-Yan Xu1

  • 1Department of Nephrology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|March 2, 2018
PubMed
Abstract

Insights

MicroRNAs miR-133a and miR-133b inhibit regulatory T cell (Treg) differentiation in IgA nephropathy by targeting FOXP3. Lower Treg percentages in IgA nephropathy patients correlate with higher miR-133a and miR-133b levels.

Area of Science:

  • Immunology
  • Molecular Biology
  • Nephrology

Background:

  • IgA nephropathy (IgAN) is a kidney disease characterized by immune dysregulation.
  • Regulatory T cells (Tregs) play a crucial role in maintaining immune tolerance.
  • The specific molecular mechanisms regulating Treg differentiation in IgAN are not fully understood.

Purpose of the Study:

  • To investigate the role of miR-133a and miR-133b in Treg differentiation in IgA nephropathy.
  • To determine if miR-133a and miR-133b target forkhead box P3 (FOXP3) to influence Treg differentiation.

Main Methods:

  • Peripheral blood mononuclear cells (PBMCs) were collected from IgAN patients and healthy controls.
  • Flow cytometry was used to quantify Treg percentages (CD4+CD25+FOXP3+).
  • Real-time PCR and western blotting assessed the expression of miR-133a, miR-133b, and FOXP3.

Main Results:

  • IgAN patients exhibited significantly lower Treg percentages compared to healthy controls.
  • miR-133a and miR-133b expression levels were significantly higher in IgAN patients.
  • Treg percentage was negatively correlated with miR-133a and miR-133b levels, and FOXP3 expression was also reduced.

Conclusions:

  • miR-133a and miR-133b inhibit Treg differentiation in IgA nephropathy.
  • These microRNAs target FOXP3, a key transcription factor for Treg function.
  • The findings suggest a novel mechanism contributing to immune dysregulation in IgAN.
Keywords:
FOXP3IgA nephropathyTreg differentiationmiR-133a/133b

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