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Elevated HPV16 E1 Expression Is Associated with Cervical Cancer Progression.

Fern Baedyananda1, Arkom Chaiwongkot2, Parvapan Bhattarakosol2

  • 1Joint PhD Program in Biomedical Sciences and Biotechnology, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

Intervirology
|March 2, 2018
PubMed
Summary

Human papillomavirus (HPV) E1 protein expression increases with cervical cancer progression. E1 mRNA levels and promoter methylation show potential as prognostic markers for cervical cancer.

Keywords:
Cervical cancerHPV E1Human papillomavirusPromoter methylation

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Area of Science:

  • Oncology
  • Virology
  • Molecular Biology

Background:

  • The human papillomavirus (HPV) E1 protein is crucial for viral DNA replication.
  • HPV E1 disrupts host cell cycle regulation and immune responses, contributing to cancer development.
  • Understanding HPV E1 expression is key to deciphering cervical cancer pathogenesis.

Purpose of the Study:

  • To investigate the expression profile of HPV16 E1 across different stages of cervical cancer.
  • To identify factors influencing HPV E1 expression, including promoter methylation and genomic variations.

Main Methods:

  • Analysis of 124 HPV16-positive cervical samples (normal, CIN 1, CIN 2/3, SCC).
  • Quantification of E1 mRNA using droplet digital PCR (ddPCR).
  • Assessment of p97 and p670 promoter methylation via pyrosequencing.
  • Determination of HPV16 E1 genome physical state and variations using PCR, qPCR, and sequencing.

Main Results:

  • E1 mRNA expression significantly increased with cervical cancer progression (normal to SCC).
  • Promoter methylation of p97 and p670 was significantly elevated in SCC compared to normal samples.
  • No correlation was found between the physical state of the HPV16 genome and E1 expression.

Conclusions:

  • HPV E1 expression correlates with cervical cancer progression.
  • E1 mRNA levels and promoter methylation may serve as valuable prognostic biomarkers for cervical cancer.