Integrated Affinity Biosensing Platforms on Screen-Printed Electrodes Electrografted with Diazonium Salts.

Area of Science:

• Electrochemistry
• Biosensor Technology
• Materials Science

Background:

• Electrode surface modification is critical for electrochemical affinity biosensor performance.
• Electrografting via diazonium salt reduction is a versatile, rapid, and simple functionalization method.
• This technique allows stable and reproducible covalent immobilization of biomolecules and nanomaterials.

Purpose of the Study:

• To review the potential of diazonium chemistry for creating electrochemical affinity biosensors.
• To highlight the application of this chemistry on screen-printed electrodes (SPEs).
• To discuss current challenges and future research directions in this field.

Main Methods:

• Utilizing diazonium salt reduction for electrode surface functionalization.
• Covalently immobilizing biomolecules and/or nanomaterials onto conductive substrates.
• Developing single or multianalyte biosensors on screen-printed electrodes (SPEs).

Main Results:

• Diazonium chemistry provides a powerful tool for stable and reproducible surface modification.
• This method enables the integration of diverse biomolecules and nanomaterials.
• The approach is suitable for fabricating electrochemical affinity biosensors on SPEs.

Conclusions:

• Diazonium chemistry is a highly promising strategy for advancing electrochemical affinity biosensor development.
• Further research is needed to address existing challenges and explore future applications.
• This functionalization method holds significant potential for single and multianalyte sensing platforms.