Related Experiment Video
Updated: Feb 13, 2026

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
Published on: January 25, 2018
Subcellular Proteomics: Application to Elucidation of Flooding-Response Mechanisms in Soybean
Setsuko Komatsu1, Akiko Hashiguchi2
1Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. komatsu.setsuko.fu@u.tsukuba.ac.jp.
Abstract:
Soybean, which is rich in protein and oil, is cultivated in several climatic zones; however, its growth is markedly decreased by flooding. Proteomics is a useful tool for understanding the flooding-response mechanism in soybean. Subcellular proteomics has the potential to elucidate localized cellular responses and investigate communications among subcellular components during plant growth and during stress. Under flooding, proteins related to signaling, stress and the antioxidative system are increased in the plasma membrane; scavenging enzymes for reactive-oxygen species are suppressed in the cell wall; protein translation is suppressed through inhibition of proteins related to preribosome biogenesis and mRNA processing in the nucleus; levels of proteins involved in the electron transport chain are reduced in the mitochondrion; and levels of proteins related to protein folding are decreased in the endoplasmic reticulum. This review discusses the advantages of a gel-free/label-free proteomic technique and methods of plant subcellular purification. It also summarizes cellular events in soybean under flooding and discusses future prospects for generation of flooding-tolerant soybean.
Related Concept Videos
Responses to Drought and Flooding
Cell-matrix's Response to Mechanical Forces
Anchoring junctions mechanically attach a cell to the...
Subcellular Fractionation
Differential Centrifugation
Differential centrifugation is...
Proteomics
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Applications of GIS: Disaster Management and Emergency Response
Design Example: Analyzing Capacity Contours for Flood Risk Assessment

