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Related Experiment Video

Updated: Feb 13, 2026

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Published on: May 26, 2013

Improving eukaryotic genome annotation using single molecule mRNA sequencing.

Vincent Magrini¹, Xin Gao¹, Bruce A Rosa¹

¹McDonnell Genome Institute, Washington University School of Medicine, St. Louis, MO, 63108, USA.

|March 3, 2018

Summary

This summary is machine-generated.

Pacific Biosciences (PacBio) single-molecule real-time sequencing significantly improved genome annotation for the hookworm Ancylostoma ceylanicum. This advanced RNA-Seq approach identified new genes and enhanced existing gene structures, including non-coding regions.

Keywords:

Ancylostoma ceylanicum Gene loci Genome annotation improvement Hookworm Pacific bioscience mRNA sequencing

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Last Updated: Feb 13, 2026

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Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Area of Science:

Genomics
Parasitology
Molecular Biology

Background:

The parasitic hookworm Ancylostoma ceylanicum poses a significant health concern.
Accurate genome annotation is crucial for understanding parasitic organisms and developing control strategies.

Purpose of the Study:

To enhance the genome annotation of Ancylostoma ceylanicum using Pacific Biosciences (PacBio) single-molecule real-time (SMRT) sequencing.
To leverage the long reads, low bias, and high accuracy of PacBio SMRT technology for improved gene discovery and characterization.

Main Methods:

Generation of 192,888 circular consensus sequences (CCS) from cDNA using the CloneTech SMARTer system.
Normalization and size-selection of SMARTer-SMRT libraries to enrich for expressed structural genes.
Application of PacBio RNA-Sequencing (RNA-Seq) for genome annotation.

Main Results:

Identification of 1609 (9.2%) new genes and extension of 3965 (26.7%) existing genes.
Increase in total genomic exon length by 1.9 Mb (12.4%) and a fifteen-fold increase in non-coding sequence representation (UTRs).
Discovery of a novel SL2 splice leader sequence for A. ceylanicum and an increase in functionally annotated genes.

Conclusions:

PacBio SMRT sequencing offers a significant improvement for genome annotation compared to conventional methods.
This technology is a valuable tool for enhancing the annotation of complex genomes, particularly in parasitic organisms.
PacBio RNA-Seq is a compelling alternative or complementary technique for transcript sequencing in genome annotation projects.