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    Area of Science:

    • Reproductive Biology
    • Molecular Biology
    • Genetics

    Background:

    • Lentiviral vectors are crucial tools for gene delivery.
    • Efficient delivery into oocytes is essential for developmental studies.

    Purpose of the Study:

    • To describe a reliable protocol for lentivirus injection into the perivitelline space of fertilized oocytes.
    • To establish a method that ensures high embryo survival rates post-injection.

    Main Methods:

    • Fertilized oocytes are prepared for microinjection.
    • Lentivirus is carefully injected into the perivitelline space.
    • Only the zona pellucida is penetrated to minimize embryo trauma.

    Main Results:

    • The described method demonstrates high embryo survival rates following lentiviral injection.
    • The protocol is effective for delivering genetic material into oocytes.

    Conclusions:

    • This protocol provides a safe and effective method for lentiviral delivery into oocytes.
    • The technique supports further research in developmental biology and gene function studies.