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Infection01:20

Infection

12.8K
When a pathogen enters the body and reproduces, it can cause an infection, damage body cells, and cause illness symptoms that eventually lead to disease. Therefore, its prevention requires breaking the chain of infection.
The chain begins with pathogens: bacteria, viruses, fungi, prions, or parasites such as protozoa helminths. These can be present on the skin as transient or resident flora, or they can be acquired from the environment. Identifying and treating the type of infection and...
12.8K
Stages of Infection01:26

Stages of Infection

65.5K
Stages of infection describe what happens to a susceptible host once a pathogen invades the human body. The stages of infection are incubation, prodromal, illness, stage of decline, and convalescence. The incubation stage is the period from exposure to a pathogen until symptoms start. The infected person is unaware of impending illness as the pathogens grow and multiply within the body. The duration may vary depending on the type of infection. The incubation period of measles averages ten to...
65.5K
Defense Mechanism Against Infection01:26

Defense Mechanism Against Infection

9.8K
Natural flora, body system defenses, and inflammation are natural barriers of the body against infectious agents regardless of previous exposure. Normal floras of the human body refer to the microbial population that colonizes the skin and mucous membranes.
In addition, many body organ systems have unique defenses against infection. The skin is an intact, multilayered surface preventing invasion by microorganisms unless impaired. Mucous membranes lining the mouth, nose, and eyelids are barriers...
9.8K
Sexually Transmitted Infections01:26

Sexually Transmitted Infections

1.2K
Sexually transmitted infections (STIs) are diseases transmitted primarily through unsafe sexual interactions. Bacteria, viruses, or parasites cause them and can result in severe health complications if untreated.ChlamydiaThe bacterium Chlamydia trachomatis is responsible for the disease Chlamydia, the most common STI in the United States. This peculiar pathogen requires human cells to reproduce, residing intracellularly. The initial infection often goes unnoticed because it typically does not...
1.2K
Factors Affecting the Risk of Infection01:26

Factors Affecting the Risk of Infection

13.9K
The hosts' susceptibility to infection depends on several factors. The integrity of the skin and mucous membranes helps protect the body against microbial attacks. When the skin is altered, the chance of infection, limb loss, and even death increases.
The integrity and count of the white blood cells help the body resist pathogens and fight infection. When impaired, it reduces the body's resistance to pathogens. The acidic pH levels of the gastrointestinal, genitourinary tracts, and skin...
13.9K
Development of the Sexual Organs in the Embryo and Fetus01:15

Development of the Sexual Organs in the Embryo and Fetus

3.9K
Development of the reproductive organs in an embryo starts from a bipotential state. This means the early embryo can develop either male or female reproductive organs. The formation of these organs begins with the growth of gonadal ridges that arise from the intermediate mesoderm during the fifth week of development.
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the...
3.9K

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Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors.

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Related Experiment Video

Updated: Feb 13, 2026

Lentivirus Production
11:42

Lentivirus Production

Published on: October 2, 2009

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Infecting Mouse Embryos by Coculturing with Lentivirus.

Shirley Pease

    Cold Spring Harbor Protocols
    |March 3, 2018
    PubMed
    Summary
    This summary is machine-generated.

    Lentiviral vectors enable transgenic animal production without costly equipment. This protocol details infecting fertilized oocytes with lentivirus via coculture for efficient transgene integration.

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    Area of Science:

    • Animal biotechnology
    • Gene delivery systems
    • Molecular biology

    Background:

    • Producing transgenic animals typically requires expensive microinjection equipment.
    • Lentiviral vectors offer an alternative method for gene transfer.
    • Efficient integration of transgenes into the host genome is crucial for creating transgenic models.

    Purpose of the Study:

    • To describe a protocol for producing transgenic animals using lentiviral vectors.
    • To demonstrate the feasibility of infecting fertilized oocytes via coculture with lentivirus.
    • To provide an accessible method for transgene integration in animal models.

    Main Methods:

    • Coculturing fertilized oocytes with lentivirus.
    • Utilizing lentiviral vectors for transgene delivery.
    • Infecting mammalian embryos at the oocyte stage.

    Main Results:

    • Successful integration of transgenes into fertilized oocytes was achieved.
    • The lentivirus coculture method bypasses the need for specialized microinjection equipment.
    • This protocol provides a cost-effective approach to generating transgenic animals.

    Conclusions:

    • Lentiviral vector-mediated coculture is an effective method for producing transgenic animals.
    • This protocol simplifies the process of generating genetically modified animals.
    • The technique offers a valuable alternative for researchers lacking access to microinjection technology.