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Updated: Feb 13, 2026

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
Use of antibiotic disks to evolve drug-resistant bacteria
J Nicole Flanagan1, Todd R Steck2
1Department of Biological Sciences, Univ. of North Carolina at Charlotte, Charlotte, NC, 28223, USA.
Abstract:
The methods used to generate antibiotic-resistant bacterial strains can be labour-intensive, costly, lengthy and/or prone to plate-to-plate variation. We propose a simple, inexpensive and easily replicated method to expose bacteria to a continuous gradient of antibiotic concentration, providing an environment of positive selective pressure for evolution of antibiotic-resistant strains.
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