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Related Concept Videos

Transcription Factors02:16

Transcription Factors

82.9K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Transcription Elongation Factors02:35

Transcription Elongation Factors

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Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
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Transcription Elongation Factors02:35

Transcription Elongation Factors

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General Transcription Factors01:30

General Transcription Factors

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
7.2K
Compounds Essential to Human Function01:25

Compounds Essential to Human Function

10.5K
The human body is composed of cells that are fundamentally made up of several different molecules. These molecules are essential to carry out all physiological processes in the body and are broadly classified into organic and inorganic based on their chemical structures.
Inorganic Compounds Essential to Human Functioning
Inorganic compounds essential to human functioning include water, salts, acids, and bases. These compounds are inorganic, i.e., they do not have a carbon-hydrogen bond. Water...
10.5K
Transcription01:10

Transcription

157.1K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
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Related Experiment Video

Updated: Feb 13, 2026

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
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Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

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The transcription factor Dach1 is essential for podocyte function.

Nicole Endlich1, Felix Kliewe1, Frances Kindt1

  • 1Department of Anatomy and Cell Biology, University Medicine Greifswald, Greifswald, Germany.

Journal of Cellular and Molecular Medicine
|March 3, 2018
PubMed
Summary

Dach1 is crucial for kidney health. This transcription factor promotes podocyte differentiation, and its loss is linked to chronic kidney disease and diabetic nephropathy progression.

Keywords:
Dach1dachddifferentiationparietal epithelial cellspodocytestranscription factors

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Area of Science:

  • Nephrology
  • Molecular Biology
  • Developmental Biology

Background:

  • Podocyte dedifferentiation and loss are primary drivers of chronic kidney disease (CKD).
  • Genome-wide association studies link the transcription factor Dach1 to glomerular filtration rate.
  • Podocytes express high Dach1 levels in vivo, but significantly lower levels in vitro.

Purpose of the Study:

  • To investigate the role of Dach1 in podocyte differentiation and kidney function.
  • To explore Dach1's potential as a therapeutic target for kidney diseases.

Main Methods:

  • In vitro studies involving transfection of parietal epithelial cells (PECs) with Dach1.
  • Immunocytochemistry and Western blot analysis to detect synaptopodin expression.
  • Zebrafish (Danio rerio) model for in vivo studies using morpholino-induced knockdown of Dachd.
  • Analysis of human diabetic nephropathy patient biopsies.

Main Results:

  • Dach1 transfection in PECs induced synaptopodin expression, a marker of podocyte differentiation.
  • Differentiating podocytes in culture showed reduced Dach1 and synaptopodin expression.
  • Zebrafish Dachd knockdown resulted in severe renal defects, including glomerular changes and filtration barrier leakage.
  • Reduced Dach1 and synaptopodin expression were observed in glomeruli from diabetic nephropathy patients compared to controls.

Conclusions:

  • Dach1 is essential for maintaining podocyte differentiation and overall kidney function.
  • Downregulation of Dach1 is associated with kidney disease progression, including diabetic nephropathy.
  • Dach1 represents a potential therapeutic target for preserving kidney health.