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Related Experiment Video

Updated: Feb 13, 2026

A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells

A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells

Published on: November 12, 2019

In vitro immunotherapy potency assays using real-time cell analysis.

Fabio Cerignoli¹, Yama A Abassi¹, Brandon J Lamarche¹

¹ACEA Biosciences, San Diego, California, United States of America.

|March 3, 2018

Summary

This summary is machine-generated.

A new real-time assay using cellular impedance monitors cancer cell killing by immune cells, enabling rapid efficacy testing of immunotherapies like CAR-T and checkpoint inhibitors.

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Area of Science:

* Immunology
* Cancer Biology
* Biotechnology

Background:

* Cancer immunotherapy has shown remarkable clinical success but faces challenges in broad application and minimizing adverse effects.
* Novel immunotherapeutics, including cell therapies and biologics, require robust functional potency assays for efficacy testing.
* Current methods for assessing immunotherapy efficacy are often time-consuming and lack real-time quantitative data.

Purpose of the Study:

* To develop and validate a real-time, in vitro functional potency assay for cancer immunotherapy.
* To quantitatively assess the cytolytic activity of various immune effector cells and therapeutic agents against tumor cells.
* To provide a simplified and reproducible workflow for immunotherapy drug development and quality control.

Main Methods:

* Development of an xCELLigence real-time cytolytic in vitro potency assay utilizing cellular impedance measurements.
* Employing specialized microtiter plates with integrated gold microelectrodes to monitor target tumor cell viability.
* Validating the assay with various effector cells (NK92, PBMC, NK, CAR-T) and immunotherapeutics (BiTEs, PD-1 blocking antibodies).

Main Results:

* The xCELLigence assay accurately monitored target tumor cell viability in real-time during immunotherapy treatment.
* Results from the real-time assay strongly correlated with traditional endpoint assays like flow cytometry.
* Quantitative parameters such as KT50 and % cytolysis were derived, enabling direct comparison of reagent efficacy.

Conclusions:

* The xCELLigence real-time cytolytic assay provides a powerful tool for functional potency testing of cancer immunotherapeutics.
* The platform offers quantitative, reproducible, and simplified assessment of immune cell-mediated tumor cell killing.
* This assay facilitates rapid evaluation of novel immunotherapies, accelerating drug development and clinical translation.