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DNAzyme-dependent Analysis of rRNA 2’-O-Methylation
Published on: September 16, 2019
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Missing the Mark: PRDM9-Dependent Methylation Is Required for Meiotic DSB Targeting.
Rhea Kang1, Maciej J Zelazowski2, Francesca Cole1
1Department of Epigenetics and Molecular Carcinogenesis, University of Texas MD Anderson Cancer Center, Science Park, Smithville, TX 78957, USA; Program in Genetics and Epigenetics, MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Science Park, Smithville, TX 78957, USA.
Molecular Cell
|March 3, 2018
Summary
The PRDM9 protein
Area of Science:
- Genetics
- Epigenetics
- Molecular Biology
Background:
- PRDM9 protein is crucial for directing meiotic recombination hotspots.
- Meiotic recombination hotspots are epigenetically marked by histone H3 methylation.
- The precise role of PRDM9's methyltransferase activity and allele dominance in hotspot localization remains unclear.
Purpose of the Study:
- To investigate whether PRDM9's methyltransferase activity is essential for hotspot localization.
- To understand the mechanism behind the dominance of certain PRDM9 alleles in controlling hotspot distribution.
Main Methods:
- The study involved analyzing the function of PRDM9 alleles and their methyltransferase activity.
- Experimental approaches were used to assess hotspot localization in the presence of different PRDM9 variants.
Main Results:
- PRDM9's methyltransferase activity was demonstrated to be necessary for the localization of meiotic recombination hotspots.
- The methyltransferase activity of combined PRDM9 alleles was found to be additive.
- Allele dominance in hotspot distribution correlates directly with the frequency of targeted sites.
Conclusions:
- PRDM9's methyltransferase function is indispensable for establishing meiotic recombination hotspots.
- The observed dominance of specific PRDM9 alleles is explained by the additive nature of their methyltransferase activity and the frequency of their target sites.
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