On the ontogeny and interactions of phosphofructokinase in mouse tissues

The distribution and interactions of phosphofructokinase isozymes with cellular structure have been studied in the major tissues of the mouse during development. The ontogenic patterns of isozymes which were obtained were consistent with those observed for other species and are interpreted in terms of the presence of three genes and three homotetrameric forms of the enzyme (A4, B4 and C4) in the tissues of the mouse. In addition, the data provides a clear indication that interactions between the enzyme and cellular structure are appreciable in all major tissues and at all stages of development, with all isozyme types exhibiting such interactions. The significance of the study of subcellular interactions of these isozymes in contributing to a comprehensive physiological rationale for this mammalian enzyme and its multiple forms is discussed.