Related Experiment Video

Updated: Feb 13, 2026

Comparison of Three Different Methods for Determining Cell Proliferation in Breast Cancer Cell Lines
12:35

Comparison of Three Different Methods for Determining Cell Proliferation in Breast Cancer Cell Lines

Published on: September 3, 2016

20.1K

NDRG4 in gastric cancer determines tumor cell proliferation and clinical outcome

Zixi Zhang1,2, Junjun She3, Jingyi Yang4

  • 1Department of Gastroenterology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Molecular Carcinogenesis
|March 4, 2018
PubMed

Insights

NDRG4, a novel tumor suppressor, is significantly decreased in gastric cancer. Lower NDRG4 levels correlate with poor patient survival and increased cancer cell proliferation, identifying it as a potential prognostic marker.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • NDRG4 is a novel candidate tumor suppressor with limited study in human cancers.
  • Gastric cancer is a significant global health concern with a need for improved prognostic markers.

Purpose of the Study:

  • To investigate NDRG4 protein and mRNA expression in gastric cancer.
  • To determine the association between NDRG4 levels and patient outcomes.
  • To evaluate the role of NDRG4 in gastric cancer cell proliferation and apoptosis.

Main Methods:

  • Recruited 286 gastric cancer patients.
  • Utilized immunohistochemistry and real-time PCR to assess NDRG4 expression.
  • Established NDRG4 overexpression and knockdown cell lines for functional studies.

Main Results:

  • NDRG4 protein and mRNA were significantly decreased in gastric cancer tissues compared to normal adjacent tissues.
  • Lower NDRG4 expression correlated with poor tumor differentiation, invasion, metastasis, and advanced stage.
  • Decreased NDRG4 levels were associated with unfavorable disease-free and overall survival.
  • NDRG4 overexpression suppressed gastric cancer cell proliferation, while knockdown enhanced it.

Conclusions:

  • NDRG4 acts as a tumor suppressor in gastric cancer.
  • NDRG4 is a potential prognostic biomarker for gastric cancer patients.
Keywords:
NDRG4apoptosisgastric cancerprognosisproliferation

More Related Videos

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines08:42

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines

Published on: July 19, 2019

10.8K
Determining the Optimal Inhibitory Frequency for Cancerous Cells Using Tumor Treating Fields TTFields08:14

Determining the Optimal Inhibitory Frequency for Cancerous Cells Using Tumor Treating Fields TTFields

Published on: May 4, 2017

15.8K

Related Concept Videos

Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
6.1K
Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
5.2K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K
Outcomes of Glycolysis01:13

Outcomes of Glycolysis

Nearly all the energy used by cells comes from the bonds that make up complex organic compounds. These organic compounds are broken down into simpler molecules, such as glucose. As a result, cells extract energy from glucose over many chemical reactions—a process called cellular respiration.
Cellular respiration can occur aerobically (with oxygen) or anaerobically (without oxygen). In the presence of oxygen, cellular respiration starts with glycolysis and continues with pyruvate...
107.8K
Gastric Motility01:16

Gastric Motility

Gastric motility is the coordinated contraction and relaxation of stomach muscles that convert ingested food into chyme, a semi-liquid substance ready for further digestion in the intestines. The process begins with the vagus nerve inducing the relaxation of the smooth muscles in the fundus and body of the stomach, allowing these regions to expand and accommodate up to approximately 1.5 liters of food and liquid.
Peristaltic Waves and Chyme Formation
Upon food entry, the stomach initiates...
3.3K
Predicting Reaction Outcomes02:24

Predicting Reaction Outcomes

Kinetics describes the rate and path by which a reaction occurs. In contrast, thermodynamics deals with state functions and describes the properties, behavior, and components of a system. It is not concerned with the path taken by the process and cannot address the rate at which a reaction occurs. Although it does provide information about what can happen during a reaction process, it does not describe the detailed steps of what appears on an atomic or a molecular level. On the other hand,...
11.0K