Related Experiment Video
Updated: Feb 13, 2026
04:55
Assessment of DNase Activity by Ratiometric Fluorescence Resonance Energy Transfer
Published on: July 25, 2025
755
A ratiometric fluorescence probe for lysosomal polarity
Miao Li1, Jiangli Fan1, Haidong Li1
1State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Rd., Hi-tech Zone, Dalian 116024, PR China.
Biomaterials
|March 4, 2018
Summary
Researchers developed a novel fluorescence probe, NOH, for ratiometric imaging of lysosomal polarity. This tool enables quantitative detection of lysosomal polarity, aiding in understanding cell disorders and diseases.
Area of Science:
- Cell Biology
- Biochemistry
- Molecular Imaging
Background:
- Lysosomal polarity is crucial for enzyme-substrate interactions within cells.
- Aberrant lysosomal polarity is associated with various diseases, necessitating advanced detection methods.
Purpose of the Study:
- To develop the first ratiometric fluorescence probe for quantitative imaging of lysosomal polarity.
- To establish a novel tool for investigating lysosome-related physiological and pathological processes.
Main Methods:
- Synthesis and characterization of the NOH fluorescence probe.
- Ratiometric fluorescence imaging in MCF-7 cells and under conditions of lysosomal storage disorders.
- Quantitative analysis of lysosomal polarity using Boltzmann function and fluorescence intensity ratios.
Main Results:
- The NOH probe exhibits specific lysosomal localization (Rr = 0.97) and dual-peak emission.
- NOH shows a Boltzmann function response of fluorescence intensity ratio to polarity.
- Lysosomal polarity in MCF-7 cells was quantitatively determined as 0.224.
Conclusions:
- The NOH probe is a promising tool for real-time, quantitative assessment of lysosomal polarity.
- This method allows for the detection of changes in lysosomal polarity associated with disease states.
- NOH facilitates deeper understanding of lysosome function in health and disease.
Related Concept Videos
01:31Lysosomes
26.5K
Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...
26.5K
01:01Group Polarization
39.2K
Group polarization is the strengthening of an original group attitude following the discussion of views within a group (Teger & Pruitt, 1967). That is, if a group initially favors a viewpoint, after discussion the group consensus is likely a stronger endorsement of the viewpoint. Conversely, if the group was initially opposed to a viewpoint, group discussion would likely lead to stronger opposition.
39.2K
01:22Lysosomal Hydrolases
4.6K
Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
4.6K
03:37Molecular Shape and Polarity
76.1K
Dipole Moment of a Molecule
76.1K
01:36Delivery Pathways to the Lysosome
10.3K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
10.3K
01:24Polar Coordinates
386
The polar coordinate system offers an alternative to the Cartesian coordinate system for specifying points in a plane, using a distance and an angle instead of x and y coordinates. This system is particularly advantageous in situations involving circular or rotational symmetry, such as in physics or engineering problems involving waves, oscillations, or orbital paths.Defining Polar CoordinatesIn polar coordinates, a point is represented as P(r, ��), where r is the radial distance...
386