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Core-shell assay based aptasensor for sensitive and selective thrombin detection using dark-field microscopy.

Rui Yang1, Shuwen Liu2, Zhenjie Wu3

  • 1Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, People's Republic of China.

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Summary
This summary is machine-generated.

This study presents a new biosensor for detecting thrombin using gold nanoparticles (AuNPs) and dark field microscopy (DFM). The sensor offers ultrasensitive detection with a low limit for thrombin quantification.

Keywords:
AptasensorAuNPsDFMThrombin

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Ultrasensitive detection of biomarkers is crucial for early disease diagnosis.
  • Existing methods often involve complex procedures and expensive equipment.
  • Aptasensors offer a promising alternative due to their specificity and ease of use.

Purpose of the Study:

  • To develop a robust and ultrasensitive aptasensor for thrombin detection.
  • To utilize microscopic enumeration of gold nanoparticles (AuNPs) for quantitative analysis.
  • To establish a simple and cost-effective diagnostic tool.

Main Methods:

  • Fabrication of a core-shell aptasensor using magnetic beads (MBs), aptamers, and AuNPs.
  • Target-induced release of AuNPs from MBs upon thrombin binding.
  • Quantification of released AuNPs using dark field microscopy (DFM) and UV-Vis spectroscopy.

Main Results:

  • The aptasensor demonstrated ultrasensitive detection of thrombin with a limit of detection of 2.54 µM and a dynamic range of 6 µM-100 µM.
  • Linear correlation between thrombin concentration and released AuNPs was observed within the dynamic range.
  • UV-Vis spectroscopy provided convenient quantification up to 100 nM, with a color change to purple at higher concentrations.

Conclusions:

  • The developed aptasensor is robust, ultrasensitive, and easy to operate.
  • The strategy is versatile and can be adapted for detecting other biomarkers.
  • This method shows significant potential for clinical diagnostics with minimal instrumentation.