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Updated: Feb 13, 2026
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
Published on: February 9, 2012
A small molecular pH-dependent fluorescent probe for cancer cell imaging in living cell
Junbao Ma1, Wenqi Li2, Juanjuan Li2
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Jiangsu Key Laboratory of Advanced Organic Materials, Nanjing University, Nanjing 210093, People's Republic of China.
Abstract:
A novel pH-dependent two-photon fluorescent molecular probe ABMP has been prepared based on the fluorophore of 2, 4, 6-trisubstituted pyridine. The probe has an absorption wavelength at 354 nm and corresponding emission wavelength at 475 nm with the working pH range from 2.20 to 7.00, especially owning a good liner response from pH = 2.40 to pH = 4.00. ABMP also has excellent reversibility, photostability and selectivity which promotes its ability in analytical application. The probe can be excited with a two-photon fluorescence microscopy and the fluorescence cell imaging indicated that the probe can distinguish Hela cancer cells out of normal cells with a two-photon fluorescence microscopy which suggested its potential application in tumor cell detection.
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