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Updated: Feb 13, 2026

Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
Published on: July 14, 2017
A miniaturized sorbent phase-based extraction device in the form of syringe filter holder using molecularly imprinted
Xiao Sun1, Xin-Yue Jiao1, Jing Li1
1Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, China.
Abstract:
The molecularly imprinted polymer using 2,4-dihydroxybenzophenone as the template (DHBP-MIP) was synthesized via sacrificial support method. The DHBP-MIP was demonstrated to possess good adsorption capacity and selectivity towards benzophenones. Moreover, a miniaturized sorbent phase-based extraction device in the form of syringe filter holder using DHBP-MIP as the sorbent was proposed, and named as μ-SPE-SFH-MIP device. The μ-SPE-SFH-MIP device consisted of a reusable syringe filter holder, flexible amount of sorbent and a sub-microporous membrane, which could be conveniently connected to different driving forces, like syringe pump, solid-phase extraction device and peristaltic pump. It was successfully applied to extract five benzophenones from swimming pool water and human urine samples, combined with high performance liquid chromatograph-UV analysis, with detection limits of 0.12-0.68 μg L-1 and 0.21-1.05 μg L-1, respectively. The accuracy and precision of the analytes were in the range of 89.27%-113.35% for swimming pool water samples and 90.65%-108.00% for urine samples, with all the relative standard deviation values below 7.89%. The μ-SPE-SFH-MIP device was portable, cost-effective operational convenient and suitable for the small-size samples; moreover, with the MIP as the sorbent, it afforded additional high selectivity and sensitivity.
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