Related Experiment Video
Updated: Feb 13, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
Label-free and simple detection of endotoxins using a sensitive LSPR biosensor based on silver nanocolumns
Mohamad Zandieh1, Seyed Nezamedin Hosseini2, Manouchehr Vossoughi3
1Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran; Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran.
Abstract:
This paper describes the construction of a silver-based LSPR biosensor for endotoxin detection. We used GLAD method to procure reproducible silver nanocolumns. In this work, the silver nanostructures were considerably stabilized by a SAM of MPA, and the limit of detection of biosensor was measured to be 340 pg/ml for endotoxin E. coli. Considering endotoxin B. abortus as the second type of endotoxin contamination in our target samples (HBs-ag produced in Institute Pasteur, Iran), we investigated selectivity of the biosensor in various experiments. We showed that this biosensor can selectively detect both types of endotoxins compared to other biological species. Overall, this study proposes that LSPR biosensing can be considered as a sensitive, simple, and label-free method for endotoxin detection in the quality control laboratories.
Related Concept Videos
Simple Pendulum
The period of a simple pendulum depends on two factors: its length and the acceleration due to gravity. The period is completely independent of any other factors, such as mass or maximum displacement. For small displacements, a pendulum is...
Simple Trusses
The most basic planar truss is a simple truss with three members arranged in a triangular formation. This triangular truss is inherently stable and rigid due to its geometry, making it an ideal starting point for creating more...
Sensitivity, Specificity, and Predicted Value
Sensitivity is the...
Simple Harmonic Motion
Labeling Emotion
Simple Staining Technique

