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Related Concept Videos

RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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RNA Splicing01:32

RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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RNA Stability01:53

RNA Stability

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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

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Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
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RNA Editing02:23

RNA Editing

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

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RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
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Updated: Feb 13, 2026

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
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Native RNA-Sequencing Throws its Hat into the Transcriptomics Ring.

Shobbir Hussain1

  • 1Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, BA2 7AY, UK.

Trends in Biochemical Sciences
|March 6, 2018
PubMed
Summary
This summary is machine-generated.

Researchers developed native RNA sequencing, a new method that bypasses DNA intermediates. This technique offers a more accurate view of native RNA molecules, improving transcriptome analysis.

Keywords:
MinIONONTOxford Nanopore TechnologiesRNA-Seqalternative splicingepitranscriptomeepitranscriptomicsnanopore sequencing

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Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • Traditional transcriptome sequencing relies on DNA conversion, which can introduce biases and inaccuracies.
  • Existing methods may not fully represent the native RNA composition due to the DNA intermediate.
  • Accurate transcriptome profiling is crucial for understanding gene expression and cellular function.

Purpose of the Study:

  • To introduce and validate a novel method for direct, native RNA sequencing.
  • To overcome the limitations associated with DNA-based transcriptome sequencing.
  • To provide a more accurate and comprehensive approach to transcriptomics.

Main Methods:

  • Development of a highly parallel, native RNA sequencing platform.
  • Direct sequencing of RNA molecules without conversion to complementary DNA (cDNA).
  • Application of the native RNA sequencing method to transcriptome surveys.

Main Results:

  • Demonstrated the feasibility of highly parallel native RNA sequencing.
  • Showcased the potential for more accurate transcriptome composition analysis.
  • Presented a new tool with potential to advance transcriptomics.

Conclusions:

  • Native RNA sequencing offers a significant advancement over traditional methods.
  • This technology has the potential to disrupt the field of transcriptomics.
  • Future applications may lead to a deeper understanding of RNA biology.