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Updated: Feb 13, 2026
07:34
Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
Published on: March 14, 2013
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Metabolomics Based Profiling of Dexamethasone Side Effects in Rats
Abeer K Malkawi1,2, Karem H Alzoubi1, Minnie Jacob3,4
1Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan.
Frontiers in Pharmacology
|March 6, 2018
Summary
Dexamethasone treatment in rats caused significant weight loss, hyperglycemia, and altered amino acid and lipid metabolism. Metabolomics profiling revealed changes linked to side effects, suggesting potential for predicting and intervening in adverse drug reactions.
Area of Science:
- Biochemistry
- Pharmacology
- Metabolomics
Background:
- Dexamethasone (Dex) is a synthetic glucocorticoid with anti-inflammatory and immunosuppressant properties.
- Long-term or high-dose Dex can cause side effects like hyperglycemia, weight changes, and osteoporosis due to non-selectivity.
- Understanding Dex-induced metabolic changes is crucial for managing its adverse effects.
Purpose of the Study:
- To investigate the side effects of Dexamethasone treatment in rats using metabolomic profiling and radiographic imaging.
- To identify specific metabolic alterations associated with Dexamethasone therapy.
- To explore the potential of metabolomics in predicting and managing Dexamethasone-related adverse events.
Main Methods:
- Liquid chromatography-tandem mass spectrometry-based comprehensive targeted metabolomic profiling.
- Radiographic imaging techniques.
- Analysis of serum metabolites, including amino acids, lipids, and acylcarnitines in Dexamethasone-treated rats compared to controls.
Main Results:
- Dexamethasone-treated rats exhibited reduced weight gain (∼20%), hyperglycemia (145 mg/dL), and decreased serum alkaline phosphatase (ALP) (∼600 IU/L).
- Distinct metabolomic profile observed, including reduced phenylalanine, lysine, arginine, and elevated tyrosine and hydroxyproline, indicating altered gluconeogenesis and protein catabolism.
- Marked elevation in sorbitol and very long-chain acylcarnitines, alongside potential inhibition of hepatic fumarylacetoacetate hydrolase, suggesting polyol pathway activation and adipose tissue degradation.
Conclusions:
- Long-term Dexamethasone therapy in rats is associated with a characteristic metabolic profile that correlates with observed side effects.
- Metabolomics profiling can identify specific biochemical perturbations induced by Dexamethasone.
- Metabolomics may offer a predictive tool for Dexamethasone treatment-related side effects and guide novel therapeutic interventions.
Keywords:
dexamethasoneglucocorticoidsmass spectrometrymetabolomicsosteoporosispharmacometabolomicsratsside-effectsMore Related Videos
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