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Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo
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Distinct Profiles of CD163-Positive Macrophages in Idiopathic Interstitial Pneumonias

Masahiro Yamashita1, Ryoko Saito2, Shinji Yasuhira3

  • 1Department of Pulmonary Medicine, Allergy and Rheumatology, Iwate Medical University School of Medicine, Morioka, Japan.

Journal of Immunology Research
|March 7, 2018
PubMed
Abstract

Insights

CD163-positive macrophages differ across idiopathic interstitial pneumonias (IIPs). Their numbers are lower in idiopathic pulmonary fibrosis (IPF), a condition with a poor prognosis, suggesting a role in disease progression.

Area of Science:

  • Pulmonary Medicine
  • Immunohistochemistry
  • Cell Biology

Background:

  • The cellular drivers of idiopathic interstitial pneumonias (IIPs) subtypes are not fully understood.
  • Limited research exists on CD163-positive macrophages in IIPs.

Purpose of the Study:

  • To immunohistochemically analyze CD163-positive macrophages in various IIPs.
  • To compare macrophage profiles across different IIP subtypes and controls.

Main Methods:

  • Retrospective analysis of lung tissue from 47 IIP patients (IPF, NSIP, COP) and 12 controls.
  • Immunohistochemistry using CD68 (pan macrophage) and CD163 (M2 macrophage) antibodies.

Main Results:

  • CD68-positive macrophage density increased in all IIPs compared to controls.
  • CD163-positive macrophage density was higher in NSIP and COP than in IPF.
  • The ratio of CD163/CD68 macrophages was lower in IPF/UIP compared to other IIPs and controls.

Conclusions:

  • Distinct CD163-positive macrophage profiles exist among IIP subtypes.
  • Reduced CD163-positive macrophage density in IPF correlates with poor prognosis.

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