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[Bradykinin stimulates prolactin release in vitro].
Comptes Rendus Des Seances De La Societe De Biologie Et De Ses Filiales
|January 1, 1986
Summary
Bradykinin (BK), a substance found in blood and the hypothalamus, stimulates prolactin (PRL) release from pituitary cells. This study shows BK is a potent PRL stimulator, with effects observed at low concentrations.
Area of Science:
- Neuroendocrinology
- Pharmacology
Background:
- Bradykinin (BK) is a vasoactive peptide involved in pain and inflammation.
- Recent studies have localized BK within hypothalamic neurons and fibers.
- The role of BK in regulating pituitary hormone release remained largely unexplored.
Purpose of the Study:
- To investigate the effect of Bradykinin (BK) on prolactin (PRL) release from anterior pituitary cells.
- To determine if BK is a direct stimulator of PRL secretion.
- To characterize the dose-dependency of BK-induced PRL release.
Main Methods:
- Primary anterior pituitary cells were cultured in vitro.
- Cells were treated with varying concentrations of BK (starting from 1 nM).
- Prolactin (PRL) levels in the culture medium were measured to assess release.
Main Results:
- Bradykinin (BK) significantly stimulated prolactin (PRL) release from anterior pituitary cells.
- The stimulatory effect of BK on PRL release was dose-dependent.
- Significant PRL release was observed even at a low concentration of 1 nM BK.
Conclusions:
- Bradykinin (BK) is a potent stimulator of prolactin (PRL) secretion.
- Anterior pituitary cells in culture provide a viable model for studying BK's mechanism of action.
- These findings suggest a novel role for BK in neuroendocrine regulation.