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A short-term in vivo model for Merkel Cell Carcinoma

Vishwanath Kumble Bhat1, Corinna Krump2, Eva Bernhart1

  • 1Institute of Molecular Biology and Biochemistry, Medical University of Graz, Graz, Austria.

Experimental Dermatology
|March 7, 2018
PubMed

Insights

The avian chorioallantoic membrane (CAM) assay effectively models Merkel cell carcinoma (MCC) growth and development. This study validates the CAM model as a valuable alternative for cancer research, aligning with the 3Rs principles.

Area of Science:

  • Oncology
  • Experimental Pathology
  • Animal Models

Background:

  • In vivo tumor models are crucial for cancer research but raise ethical concerns regarding animal use.
  • The 3Rs principles (replacement, refinement, reduction) drive the development of alternative experimental models.
  • The avian chorioallantoic membrane (CAM) model has emerged as a promising alternative to rodent models for cancer studies.

Purpose of the Study:

  • To evaluate the avian chorioallantoic membrane (CAM) assay as an alternative model for studying Merkel cell carcinoma (MCC).
  • To assess the feasibility of using CAM assays for investigating MCC pathophysiology, including tumor growth and metastasis.

Main Methods:

  • Three Merkel cell carcinoma (MCC) cell lines were grafted onto the avian CAM.
  • Tumor growth and the development of solid tumor nodules were monitored.
  • Xenograft morphology was characterized using histological and immunohistochemical analyses.

Main Results:

  • The CAM assay successfully supported the growth of Merkel cell carcinoma xenografts.
  • Histological and immunohistochemical analyses confirmed the development of solid tumor nodules.
  • The model allowed for the observation of MCC pathophysiology in a controlled environment.

Conclusions:

  • The avian chorioallantoic membrane (CAM) assay is a viable and useful tool for studying Merkel cell carcinoma (MCC) pathophysiology.
  • This model offers a valuable alternative to traditional animal models, supporting the 3Rs principles in cancer research.
Keywords:
Chorioallantoic membrane assayMCPyV-LT antigenMerkel cell carcinoma

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