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A Rapid and Semi-Quantitative Gold Nanoparticles Based Strip Sensor for Polymyxin B Sulfate Residues

Yue Li1,2,3, Liqiang Liu4,5,6, Shanshan Song7,8,9

  • 1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China. liyuejiangnan@163.com.

Nanomaterials (Basel, Switzerland)
|March 8, 2018
PubMed

Insights

Rapid detection of polymyxin B (PMB) residues is crucial due to increasing resistance. This study developed a sensitive gold nanoparticles-based immunochromatographic test (ICT) strip for quick PMB detection in milk and animal feed.

Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Food Safety

Background:

  • Polymyxin B (PMB) is a last-resort antibiotic for multidrug-resistant Gram-negative bacterial infections.
  • Increasing reports of polymyxin resistance pose a significant public health threat.
  • Rapid detection methods for PMB residues are essential to ensure food safety and public health.

Purpose of the Study:

  • To develop and validate a rapid, sensitive detection method for polymyxin B (PMB) residues.
  • To establish monoclonal antibodies (mAbs) for PMB detection.
  • To create a gold nanoparticles (AuNPs)-based immunochromatographic test (ICT) strip for semi-quantitative analysis.

Main Methods:

  • Production of two monoclonal antibodies (mAbs), 2A2 and 3C6, using PMB-bovine serum albumin and PMB-ovalbumin.
  • Comparison of mAb sensitivity using indirect competitive enzyme-linked immunosorbent assay (ic-ELISA).
  • Development of an AuNPs-based ICT strip utilizing the highly sensitive 3C6 mAb.

Main Results:

  • mAb 3C6 demonstrated higher sensitivity with an IC50 of 13.13 ng/mL.
  • The ICT strip showed visible detection limits of 25 ng/mL in milk and 500 μg/kg in animal feed.
  • Cutoff limits for the ICT strip were established at 100 ng/mL for milk and 1000 μg/kg for animal feed.

Conclusions:

  • A sensitive and rapid AuNPs-based ICT strip was successfully developed for polymyxin B (PMB) residue detection.
  • The ICT strip provides results within 15 minutes, enabling fast semi-quantitative analysis.
  • This method facilitates effective monitoring of PMB residues in milk and animal feed, contributing to public health and food safety.
Keywords:
animal feedgold nanoparticlesimmunochromatographic test stripmilkmonoclonal antibodypolymyxin B

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