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Updated: Feb 13, 2026

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
Published on: April 2, 2020
[Various approaches of endometrial preparation for frozen-thawed embryo transfer]
Objective:
Various approaches of endometrial preparation for frozen-thawed embryo transfer.
Design:
Retrospective analysis.
Setting:
Department of Obstetric and Gyneacology, Faculty of Medicine, Palacky University, University Hospital Olomouc.
Methods:
Effectivity of frozen-thawed embryo transfers was carried out between January and August 2017 in the IVF unit at the University Hospital Olomouc. Results were compared among groups A, B, C with various approaches of endometrial preparation. Group A - natural ovulating cycle, group B - artificial cycle with oral estrogen, vaginal gestagen and group C - artificial cycle with transdermal estrogen and vaginal gestagen. One blastocyst (two blastocyst in five cycles) was transferred on day 6 post ovulation (group A) or after 6 days of using gestagens (group B, C).
Results:
The highest pregnancy rate was observed in group C (56%), similar pregnancy rate was found in group B (52%) and the lowest was seen in group A (22%). All pregnancies in group A continued over 12 weeks, in group B continued 57 percent and in group C continued 56 percent of pregnancies.
Conclusion:
Frozen-thawed embryotransfers in natural cycles are highly time-consuming. Our study has demonstrated the lowest effectivity of frozen-embryotransfer in natural cycles. Artificial cycles managed by oral or transdermal form of estrogen have shown better results.
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