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Related Concept Videos

Nucleic Acids02:43

Nucleic Acids

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
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Nucleic acids02:43

Nucleic acids

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
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The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
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Nucleic Acids02:43

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Nucleic Acid Structure01:25

Nucleic Acid Structure

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The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
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Nucleic Acids and Nucleotides01:20

Nucleic Acids and Nucleotides

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and have instructions for its functioning. The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).
Deoxyribonucleic Acid (DNA)
DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and the organelles such as chloroplasts and mitochondria....
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Biosynthesis of Nucleic Acids01:28

Biosynthesis of Nucleic Acids

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Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
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Related Experiment Video

Updated: Feb 13, 2026

Sentinel Lymph Node Mapping and Biopsy for Endometrial Cancer at Early Stage with Laparoscopy
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Sentinel Lymph Node Mapping and Biopsy for Endometrial Cancer at Early Stage with Laparoscopy

Published on: August 19, 2021

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[One-Step Nucleic Acid Amplification method - what is the future of sentinel lymph node management?]

J Kosťun, M Pešta, R Slunečko

    Ceska Gynekologie
    |March 8, 2018
    PubMed
    Summary

    The One-Step Nucleic Acid Amplification (OSNA) method, detecting cytokeratin 19 (CK19), is effective for perioperative sentinel lymph node management in breast and colorectal cancers. Further research is needed for its application in other cancers.

    Keywords:
    CK19 sentinel lymph node.OSNA

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    Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
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    Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

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    Area of Science:

    • Oncology
    • Surgical Pathology
    • Molecular Diagnostics

    Background:

    • Sentinel lymph node biopsy is crucial for cancer staging.
    • Accurate and rapid assessment of sentinel lymph nodes is essential for perioperative management.
    • Current methods may have limitations in speed or sensitivity.

    Purpose of the Study:

    • To review the current applications of the One-Step Nucleic Acid Amplification (OSNA) method.
    • To evaluate OSNA's utility in perioperative sentinel lymph node management.
    • To assess OSNA's potential in various oncologic surgeries.

    Main Methods:

    • Literature review of studies published until April 2017.
    • Search conducted on the PubMed database using "OSNA" and "One-Step Nucleic Acid Amplification".
    • Focus on studies utilizing cytokeratin 19 (CK19) detection in sentinel lymph nodes.

    Main Results:

    • OSNA, detecting CK19, is an acceptable method for perioperative sentinel lymph node management.
    • The method is currently established for breast and colorectal cancer.
    • Preliminary data suggests potential for thyroid, gastric, uterine, and head and neck cancers.

    Conclusions:

    • OSNA is a viable option for sentinel lymph node analysis in specific cancers.
    • Expanded use in other malignancies requires further investigation and data.
    • The method offers a promising approach to improve oncologic surgical management.