Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Motion Of A Charged Particle In A Magnetic Field01:22

Motion Of A Charged Particle In A Magnetic Field

7.2K
A charged particle experiences a force when moving through a magnetic field. Consider the field to be uniform and the charged particle to move perpendicular to it. If the field is in a vacuum, the magnetic field is the dominant factor determining the motion. Since the magnetic force is perpendicular to the direction of motion, a charged particle follows a curved path. The particle continues to follow this curved path until it forms a complete circle. Another way to look at this is that the...
7.2K
Formal Charges02:42

Formal Charges

40.7K
In some cases, there are seemingly more than one valid Lewis structures for molecules and polyatomic ions. The concept of formal charges can be used to help predict the most appropriate Lewis structure when more than one reasonable structure exists.
40.7K
Ions and Ionic Charges03:27

Ions and Ionic Charges

79.5K
In ordinary chemical reactions, the nucleus — which contains the protons and neutrons of each atom and thus identifies the element — remains unchanged. Electrons, however, can be added to atoms by transfer from other atoms, lost by transfer to other atoms, or shared with other atoms. The transfer and sharing of electrons among atoms govern the chemistry of the elements. During the formation of some compounds, atoms gain or lose electrons to form electrically charged particles called...
79.5K
Subatomic Particles03:37

Subatomic Particles

114.0K
Dalton was only partially correct about the particles that make up matter. All matter is composed of atoms, and atoms are composed of three smaller subatomic particles: protons, neutrons, and electrons. These three particles account for the mass and the charge of an atom.
114.0K
Trends in Lattice Energy: Ion Size and Charge02:54

Trends in Lattice Energy: Ion Size and Charge

26.8K
An ionic compound is stable because of the electrostatic attraction between its positive and negative ions. The lattice energy of a compound is a measure of the strength of this attraction. The lattice energy (ΔHlattice) of an ionic compound is defined as the energy required to separate one mole of the solid into its component gaseous ions. For the ionic solid sodium chloride, the lattice energy is the enthalpy change of the process:
26.8K
The Nucleosome Core Particle02:10

The Nucleosome Core Particle

14.6K
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
14.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Electrokinetic separation of bacteriophage ϕKZ from bacterial cells.

The Analyst·2026
Same author

Electrophoresis: An Effective Viability Assessment Method across Cell Domains.

Analytical chemistry·2026
Same author

Nonantiperiodic Nonlinear Electrophoresis of Colloidal Particles.

Analytical chemistry·2025
Same author

Climate Change, Fisheries Management, and Increases in Demersal Fish Distribution in a Southern Ocean Biodiversity Hotspot.

Global change biology·2025
Same author

Policy and preference: the intersection of attendance hurdles and student perceptions of practical classes.

BMC medical education·2025
Same author

Fifty Years of Nonlinear Electrophoresis.

Electrophoresis·2025

Related Experiment Video

Updated: Feb 13, 2026

Microfluidic Device for the Separation of Non-Metastatic MCF-7 and Non-Tumor MCF-10A Breast Cancer Cells Using AC Dielectrophoresis
08:33

Microfluidic Device for the Separation of Non-Metastatic MCF-7 and Non-Tumor MCF-10A Breast Cancer Cells Using AC Dielectrophoresis

Published on: August 11, 2022

2.9K

Separating large microscale particles by exploiting charge differences with dielectrophoresis.

Danielle V Polniak1, Eric Goodrich1, Nicole Hill1

  • 1Microscale Bioseparations Laboratory and Biomedical Engineering Department, Rochester Institute of Technology, Rochester NY, USA.

Journal of Chromatography. A
|March 8, 2018
PubMed
Summary

This study demonstrates insulator-based dielectrophoresis (iDEP) for separating larger microparticles based on subtle charge differences, overcoming limitations of traditional electrophoresis. This method offers a new approach for characterizing and separating particles, including potentially similar biological cells.

Keywords:
DielectrophoresisElectric fieldElectrical chargeElectrokineticsElectrophoresisMicroparticles

More Related Videos

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

16.7K
Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

28.1K

Related Experiment Videos

Last Updated: Feb 13, 2026

Microfluidic Device for the Separation of Non-Metastatic MCF-7 and Non-Tumor MCF-10A Breast Cancer Cells Using AC Dielectrophoresis
08:33

Microfluidic Device for the Separation of Non-Metastatic MCF-7 and Non-Tumor MCF-10A Breast Cancer Cells Using AC Dielectrophoresis

Published on: August 11, 2022

2.9K
Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

16.7K
Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

28.1K

Area of Science:

  • Electrokinetics
  • Microfluidics
  • Particle Separation

Background:

  • Electrophoresis (EP) is limited in separating larger microparticles (>5 µm) due to low electrophoretic mobilities.
  • Dielectrophoresis (DEP) offers an alternative by utilizing particle polarization in nonuniform electric fields.

Purpose of the Study:

  • To characterize and separate larger microparticles using insulator-based dielectrophoresis (iDEP).
  • To demonstrate iDEP's capability in exploiting charge differences for particle separation, particularly for particles with similar physical properties.

Main Methods:

  • Utilized microdevices for insulator-based dielectrophoresis (iDEP) to expose microparticles to DEP, EP, and electroosmotic (EO) forces simultaneously.
  • Characterized microparticle electrokinetic behavior using velocimetry and dielectrophoretic capture assessments.
  • Performed dielectropherogram separation of 10 µm polystyrene microparticles.

Main Results:

  • Successfully separated two distinct types of 10 µm microparticles in under 80 seconds using iDEP.
  • Demonstrated that iDEP can separate particles of identical size, shape, and material by exploiting minor variations in surface charge.
  • Achieved distinct elution peaks, indicating successful enrichment of separated particle types.

Conclusions:

  • Insulator-based dielectrophoresis (iDEP) provides an effective method for separating larger microparticles based on charge differences, surpassing EP limitations.
  • This technique holds potential for assessing and separating biological cells with subtle variations in surface electrical charge.
  • iDEP offers a versatile platform for microparticle characterization and separation in microfluidic devices.