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Establishing an Animal Model of Secondary Osteoporosis by Using a Gonadotropin-releasing Hormone Agonist

Nur-Vaizura Mohamad1, Muhammad Afiq Amani Che Zulkepli1, Krystine May Theseira1

  • 1Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, 56000 Cheras, Kuala Lumpur, Malaysia.

International Journal of Medical Sciences
|March 8, 2018
PubMed

Insights

Buserelin, a GnRH agonist, effectively reduces testosterone and bone microarchitecture in male rats, mimicking orchidectomy effects. Longer studies may reveal impacts on bone strength and mineral content.

Area of Science:

  • Endocrinology
  • Osteoporosis Research
  • Preclinical Animal Models

Background:

  • Orchidectomy is the standard for inducing bone loss in male osteoporosis models.
  • Gonadotropin-releasing hormone (GnRH) agonists, used in prostate cancer treatment, cause testosterone deficiency but their skeletal effects in preclinical models are less explored.

Purpose of the Study:

  • To evaluate the skeletal effects of buserelin (a GnRH agonist) in male rats.
  • To compare buserelin's skeletal effects with those of orchidectomy.

Main Methods:

  • Male Sprague-Dawley rats were divided into baseline, buserelin (25 or 75 µg/kg), and orchidectomy (or sham) groups.
  • In-vivo micro-CT scanning, blood testosterone level analysis, and femur biomechanical testing were performed over three months.

Main Results:

  • Buserelin significantly decreased testosterone levels and deteriorated bone microstructure, similar to orchidectomy.
  • Buserelin treatment affected femur biomechanical properties, while orchidectomy did not show significant changes in strength within three months.
  • Bone calcium content remained unaffected by buserelin treatment.

Conclusions:

  • Buserelin effectively induces testosterone deficiency and bone microarchitectural deterioration in rats within three months, comparable to orchidectomy.
  • Extended treatment duration might be necessary to observe significant effects on bone strength and mineral content.
Keywords:
androgenbonegonadotropin-releasing hormone agonistsmale osteoporosistestosterone

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