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Updated: Feb 13, 2026

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors
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MiR-103 regulates the angiogenesis of ischemic stroke rats by targeting vascular endothelial growth factor (VEGF)

Fu-Ping Shi1, Xue-Hong Wang2, Hong-Xin Zhang3

  • 1Department of Neurology, Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China.

Iranian Journal of Basic Medical Sciences
|March 8, 2018
PubMed
Abstract

Insights

Inhibiting microRNA-103 (miR-103) promotes blood vessel formation in ischemic stroke by increasing vascular endothelial growth factor (VEGF). This finding offers a new therapeutic target for reducing stroke damage.

Area of Science:

  • Molecular Biology
  • Neuroscience
  • Cardiovascular Biology

Background:

  • Ischemic stroke is a leading cause of disability worldwide.
  • Angiogenesis plays a critical role in recovery after ischemic stroke.
  • MicroRNAs (miRNAs) are implicated in various cellular processes, including angiogenesis.

Purpose of the Study:

  • To investigate the role of miR-103 in the angiogenesis of ischemic stroke.
  • To determine if miR-103 targets vascular endothelial growth factor (VEGF).
  • To explore the therapeutic potential of modulating miR-103 in ischemic stroke.

Main Methods:

  • Rat models of middle cerebral artery occlusion (MCAO) and oxygen-glucose deprivation (OGD) cell models were used.
  • Vascular density, tube formation, and cell migration were assessed.
  • Quantitative real-time polymerase chain reaction (qRT-PCR), Western blotting, and dual-luciferase assays were employed to analyze miR-103 and VEGF expression and their interaction.

Main Results:

  • miR-103 expression was reduced in MCAO rats.
  • Inhibition of miR-103 increased VEGF expression, improved neurological scores, reduced infarct volume, and enhanced vascular density.
  • miR-103 directly targets VEGF, leading to its downregulation.
  • Silencing VEGF reversed the pro-angiogenic effects of miR-103 inhibition.

Conclusions:

  • Inhibition of miR-103 promotes angiogenesis and reduces infarction volume in ischemic stroke by enhancing VEGF.
  • Modulating miR-103 represents a potential therapeutic strategy for ischemic stroke treatment.
Keywords:
InfarctionIschemiaMIRN103 microRNAMiddle cerebral arteryStrokeVascular endothelial-growth factor A

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