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Related Concept Videos

High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

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The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule; a molecule with an odd number of nitrogen atoms produces a molecular ion with an odd molecular weight. Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit strong molecular ion peaks, but acyclic...
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Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
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Related Experiment Video

Updated: Feb 13, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
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CSF Lipidomics Analysis: High-Resolution Mass Spectrometry Analytical Platform.

Paul L Wood1, Randall L Woltjer2

  • 1Metabolomics Unit, College of Veterinary Medicine, Lincoln Memorial University, Harrogate, TN, USA. Paul.wood@lmunet.edu.

Methods in Molecular Biology (Clifton, N.J.)
|March 8, 2018
PubMed
Summary

High-resolution mass spectrometry enables comprehensive lipid analysis in cerebrospinal fluid. This method allows for both unbiased and targeted lipidomics using a single injection.

Keywords:
CSFHigh-resolution mass spectrometryLipidomics

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Neuroscience

Background:

  • Lipids are crucial for brain function and neurological disease.
  • Cerebrospinal fluid (CSF) is a valuable biofluid for studying brain biochemistry.
  • Comprehensive lipid analysis in CSF is challenging but essential.

Purpose of the Study:

  • To present a methodology for lipidomic analysis of cerebrospinal fluid.
  • To enable both unbiased and targeted lipidomics approaches.
  • To leverage high-resolution mass spectrometry for detailed lipid characterization.

Main Methods:

  • Utilizing high-resolution mass spectrometry (HRMS).
  • Employing direct infusion techniques for sample introduction.
  • Implementing workflows for unbiased and targeted lipidomics.

Main Results:

  • HRMS provides sufficient resolution for direct infusion analysis.
  • A wide array of lipids can be detected and characterized in a single injection.
  • The presented methodology is applicable to cerebrospinal fluid samples.

Conclusions:

  • Direct infusion HRMS is a powerful tool for CSF lipidomics.
  • The methodology facilitates comprehensive and targeted lipid profiling.
  • This approach aids in understanding the role of lipids in neurological conditions.