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Updated: Feb 13, 2026

In Situ Chemotaxis Assay to Examine Microbial Behavior in Aquatic Ecosystems
Published on: May 5, 2020
Analyzing Chemotaxis and Related Behaviors of Azospirillum Brasilense
Lindsey O'Neal1, Tanmoy Mukherjee1, Gladys Alexandre1
1Biochemistry &Cellular and Molecular Biology, The University of Tennessee, Knoxville, Tennesee.
Abstract:
Bacteria of the genus A. brasilense are motile and capable of chemotaxis and aerotaxis (taxis in gradient of oxygen) using a single polar flagellum that propels the cells in aqueous environments. Responses to attractants and repellents have been described and spatial gradient assays that permit the visualization of these responses are detailed in this unit. These assays are simple and can be readily implemented with minimum set ups. © 2018 by John Wiley & Sons, Inc.
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