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Updated: Feb 13, 2026

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
Published on: May 2, 2018
Occupational exposure to asphalt fume can cause oxidative DNA damage among road paving workers
Ceylan Bal1, Erol R Ağış2, Murat Büyükşekerci2
1Department of Biochemistry, Yıldırım Beyazıt University, Ankara, Turkey.
Objectives:
We designed the present study to determine the effect of occupational exposure to asphalt fumes on oxidative status and DNA damage in road paving workers.
Methods:
Sixty road paving workers exposed to asphalt fumes and forty non-exposed control subjects were recruited. Occupational exposure to PAHs was assessed by urinary 1-hydroxypyrene (1-OHP) excretion. Serum thiol disulfide homeostasis (TDH), total oxidant status (TOS) and total antioxidant status (TAS) and urinary 8-hydro-deoxyguanosine (8-OH-dG) level were evaluated by automated colourimetric method.
Results:
The urinary concentrations of 1-OHP and 8-OH-dG were significantly higher in the exposed group than in the control group (P < 0.001). Disulfide/thiol ratio, TOS, and TAS were also significantly higher for the asphalt workers. A positive correlation existed between urinary 1-OHP and 8-OH-dG, TOS and TAS.
Conclusion:
Study results indicate that exposure to PAHs induces oxidative stress and causes genotoxic effects in asphalt workers.
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