Related Experiment Video
Updated: Feb 13, 2026

Particle Agglutination Method for Poliovirus Identification
Published on: April 20, 2011
Characterisation of agglutination properties of myelin basic protein
S Basu1, G Gupta1, R G Mahajan1
1Department of Fundamental Biochemistry, n Institute of Chemical Biology, Calcutta - 700 032, India.
Abstract:
Myelin basic protein (MBP) from one month old rat brain was purified by CM-52 cellulose chromatography and heparin sepharose chromatography. It's lectin properties were studied and conditions for optimum agglutination were observed. MBP behaved likea heparin binding lectin. Carbohydrade binding specificity studies showed that on molar basis MBP was feebly inhibited by lactose and strongly inhibited by heparin. Inhibition due to heparin was not due to electrostatic forces as evidenced by no inhibition due to other glycosaminoglycans. Argine and lysine residues were involved in the agglutination activity of MBP.
Related Concept Videos
Blood Transfusion and Agglutination
History
The history of blood transfusion dates back to the 17th century, when early attempts were made in animals. In 1818 James Blundell, a British doctor, performed the first successful human blood transfusion. Later in 1900, Karl...
Energy Basics
Nervous Tissue: Myelin
Schwann cells begin to form myelin sheaths around axons during fetal development. They wrap around a small...
Basicity of Aliphatic Amines
To measure the basicity of amines, two conventions are generally used. The first defines Kb as the basicity constant for the deprotonation reaction of water by the amine, as presented in Figure 1. Conventionally, lower Kb indicates higher...
Basic Operations on Signals
Time Reversal mirrors a continuous-time signal about the vertical axis at t=0. This is achieved by substituting t with −t. For example, if a signal x(t) is considered, the time-reversed signal is x(−t). This operation can be graphically represented, showing the mirrored signal.
VSEPR Theory and the Basic Shapes

