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SOX6 is downregulated in osteosarcoma and suppresses the migration, invasion and epithelial-mesenchymal transition

Zheng Wang1, Junjie Li1, Kun Li2

  • 1Department of Hand and Foot Surgery, Changyi People's Hospital, Changyi, Shandong 261300, P.R. China.

Molecular Medicine Reports
|March 8, 2018
PubMed

Insights

Transcription factor SOX6 is downregulated in osteosarcoma (OS) and suppresses tumor growth, migration, and invasion. This study identifies SOX6 as a potential therapeutic target for OS.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Transcription factor SOX6 (SOX6) plays roles in various cancers.
  • The expression and function of SOX6 in osteosarcoma (OS) remain uninvestigated.

Purpose of the Study:

  • To investigate the expression patterns and functional roles of SOX6 in osteosarcoma.
  • To determine if SOX6 acts as a tumor suppressor in OS and explore its therapeutic potential.

Main Methods:

  • Reverse transcription-quantitative polymerase chain reaction and western blotting were used to assess SOX6 expression in OS tissues and cell lines.
  • Gain- and loss-of-function studies, chromatin immunoprecipitation (ChIP), quantitative ChIP, and dual luciferase activity assays were employed to analyze SOX6's functional impact and molecular mechanisms.
  • Colony formation and Cell Counting Kit-8 assays were conducted to evaluate SOX6's effect on cell proliferation.

Main Results:

  • SOX6 expression was significantly downregulated in OS tissues and cell lines.
  • SOX6 overexpression inhibited OS cell migration and invasion, potentially by suppressing epithelial-mesenchymal transition via modulation of twist-related protein 1 (TWIST1).
  • SOX6 was confirmed to bind the promoter region of TWIST1, and SOX6 suppressed osteosarcoma cell proliferation.

Conclusions:

  • SOX6 functions as a tumor suppressor in osteosarcoma.
  • SOX6 represents a potential therapeutic target for osteosarcoma treatment.
Keywords:
transcription factor SOX6epithelial-mesenchymal transitionmigrationinvasionproliferationosteosarcoma

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