Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Angular Momentum: Single Particle01:10

Angular Momentum: Single Particle

7.9K
Angular momentum is directed perpendicular to the plane of the rotation, and its magnitude depends on the choice of the origin. The perpendicular vector joining the linear momentum vector of an object to the origin is called the “lever arm.” If the lever arm and linear momentum are collinear, then the magnitude of the angular momentum is zero. Therefore, in this case, the object rotates about the origin such that it lies on the rim of the circumference defined by the lever arm...
7.9K
Principle of Linear Impulse and Momentum for a Single Particle01:20

Principle of Linear Impulse and Momentum for a Single Particle

1.6K
Linear momentum is a fundamental concept in physics that describes the motion of an object. It is a vector quantity, having a magnitude equal to the product of its mass and its velocity, and direction along the object's velocity. On the other hand, linear impulse, also known as momentum impulse, is a concept in physics related to the change in the linear momentum of an object. Impulse is a vector quantity defined as the product of force and the time over which the force is applied.
Delving...
1.6K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.9K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.9K
Principle of Linear Impulse and Momentum for a Single Particle: Problem Solving01:23

Principle of Linear Impulse and Momentum for a Single Particle: Problem Solving

1.1K
Consider a wooden box and a cylinder of known masses m1 and m2, respectively,  hanging from a ceiling with the help of a massless pulley system.
1.1K
Subatomic Particles03:37

Subatomic Particles

114.0K
Dalton was only partially correct about the particles that make up matter. All matter is composed of atoms, and atoms are composed of three smaller subatomic particles: protons, neutrons, and electrons. These three particles account for the mass and the charge of an atom.
114.0K
The Nucleosome Core Particle02:10

The Nucleosome Core Particle

14.6K
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
14.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Visualization of the complete preprimosome reveals the structural mechanisms governing DNA replication restart.

Nature communications·2026
Same author

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

eLife·2026
Same author

Complete inhibition of β-tryptase by tetramer dissociation and active site allostery due to a single antibody residue.

Nature communications·2026
Same author

Nodavirus protein A's interdomain elbow controls RNA replication organelle formation and function.

Nucleic acids research·2026
Same author

Visualization of the complete primosome reveals the structural mechanisms governing DNA replication restart.

bioRxiv : the preprint server for biology·2026
Same author

Atlas of lysine acetylation in the mouse.

bioRxiv : the preprint server for biology·2026
Same journal

The exquisite mechanics of a tsetse bite.

eLife·2026
Same journal

Distinct involvements of the subthalamic nucleus subpopulations in reward-biased decision-making in monkeys.

eLife·2026
Same journal

Pink1-mediated mitophagy in the endothelium releases proteins encoded by mitochondrial DNA and activates neutrophil responses during inflammation.

eLife·2026
Same journal

Restraint of melanoma progression by cells in the local skin environment.

eLife·2026
Same journal

Brawn before bite in endemic Asian eutherian mammals after the end-Cretaceous extinction.

eLife·2026
Same journal

Experimental evolution to thermal stress indicates climate resilience in a cosmopolitan arthropod.

eLife·2026
See all related articles

Related Experiment Video

Updated: Feb 13, 2026

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
07:56

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

Published on: July 29, 2021

4.0K

cisTEM, user-friendly software for single-particle image processing.

Timothy Grant1, Alexis Rohou1, Nikolaus Grigorieff1

  • 1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.

Elife
|March 8, 2018
PubMed
Summary
This summary is machine-generated.

We developed cisTEM, open-source software for transmission electron microscopy data processing. This tool enables high-resolution cryo-EM single-particle analysis on standard workstations efficiently.

Keywords:
cistemcryo-EMelectron microscopyimage analysismolecular biophysicsnonesingle-particle analysissoftwarestructural biology

More Related Videos

High-throughput Image Analysis of Tumor Spheroids: A User-friendly Software Application to Measure the Size of Spheroids Automatically and Accurately
08:39

High-throughput Image Analysis of Tumor Spheroids: A User-friendly Software Application to Measure the Size of Spheroids Automatically and Accurately

Published on: July 8, 2014

26.0K
A User-friendly and Powerful R Analysis of Large-scale Datasets
10:56

A User-friendly and Powerful R Analysis of Large-scale Datasets

Published on: November 4, 2025

393

Related Experiment Videos

Last Updated: Feb 13, 2026

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
07:56

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

Published on: July 29, 2021

4.0K
High-throughput Image Analysis of Tumor Spheroids: A User-friendly Software Application to Measure the Size of Spheroids Automatically and Accurately
08:39

High-throughput Image Analysis of Tumor Spheroids: A User-friendly Software Application to Measure the Size of Spheroids Automatically and Accurately

Published on: July 8, 2014

26.0K
A User-friendly and Powerful R Analysis of Large-scale Datasets
10:56

A User-friendly and Powerful R Analysis of Large-scale Datasets

Published on: November 4, 2025

393

Area of Science:

  • Structural Biology
  • Biophysics
  • Computational Biology

Background:

  • Transmission electron microscopy (TEM) is crucial for determining molecular structures.
  • High-resolution cryo-electron microscopy (cryo-EM) and single-particle averaging (SPA) demand robust computational tools.
  • Existing software pipelines can be resource-intensive and complex to operate.

Purpose of the Study:

  • To introduce cisTEM, a new open-source software package for processing cryo-EM data.
  • To provide a user-friendly interface for complex cryo-EM data analysis workflows.
  • To achieve efficient and high-resolution results comparable to GPU-accelerated methods.

Main Methods:

  • Development of cisTEM, featuring a graphical user interface for job submission, monitoring, and results visualization.
  • Implementation of a comprehensive processing pipeline: movie processing, defocus determination, particle picking, 2D/3D classification, and 3D reconstruction.
  • Optimization for processing typical datasets on CPU-based workstations within hours, with cluster compatibility.

Main Results:

  • cisTEM successfully processes large cryo-EM datasets.
  • The software achieves processing speeds comparable to GPU-accelerated methods on standard workstations.
  • It offers a complete pipeline from raw micrographs to high-resolution 3D maps.

Conclusions:

  • cisTEM provides an efficient, accessible, and comprehensive solution for high-resolution cryo-EM data processing.
  • The open-source nature and performance make it a valuable tool for the structural biology community.
  • It democratizes advanced cryo-EM analysis by running effectively on widely available hardware.