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Optimizing Attachment of Human Mesenchymal Stem Cells on Poly(&#949;-caprolactone) Electrospun Yarns
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Estradiol-Loaded Poly(ε-caprolactone)/Silk Fibroin Electrospun Microfibers Decrease Osteoclast Activity and Retain

Chris Steffi1, Dong Wang1,2, Chee Hoe Kong1

  • 1Department of Orthopaedic Surgery , National University of Singapore , Singapore 119074 , Singapore.

ACS Applied Materials & Interfaces
|March 8, 2018
PubMed
Summary
This summary is machine-generated.

This study developed 17-ß estradiol (E2)-loaded poly(ε-caprolactone)/silk fibroin microfibers for osteoporosis treatment. The E2-loaded microfibers effectively reduced osteoclast activity while preserving osteoblast function, showing potential for treating osteoporotic fractures.

Keywords:
electrospinningestradiolosteoblastosteoclastosteoporosispolycaprolactonesilk fibroin

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Area of Science:

  • Biomaterials Science
  • Polymer Science
  • Cell Biology

Background:

  • Estrogen deficiency accelerates osteoclast activity, contributing to osteoporosis and fractures in elderly women.
  • Localized hormone delivery systems are sought for targeted osteoporosis treatment.

Purpose of the Study:

  • To develop and evaluate 17-ß estradiol (E2)-loaded poly(ε-caprolactone)/silk fibroin (PCL/SF) electrospun microfibers as a localized E2 delivery system for osteoporotic fracture treatment.

Main Methods:

  • PCL/SF blends were electrospun and characterized. The optimal PCL50/SF50 ratio was selected and loaded with E2 at various concentrations.
  • In vitro studies assessed sustained E2 release, osteoclast activity (tartrate-resistant acid phosphatase, DNA, multinucleated cells), and osteoblast function (alkaline phosphatase, collagen I).

Main Results:

  • The PCL50/SF50 blend supported proliferation of both preosteoblasts and preosteoclasts.
  • Sustained E2 release was observed for up to 3 weeks, particularly at higher concentrations.
  • E2-loaded microfibers significantly reduced osteoclast activity markers while maintaining osteoblast alkaline phosphatase activity and collagen I expression.

Conclusions:

  • The developed E2-loaded PCL/SF electrospun microfibers demonstrate potential as a localized delivery system for treating osteoporotic fractures.
  • This system effectively modulates bone cell activity, offering a promising therapeutic strategy for estrogen deficiency-related bone loss.