Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Structural Protein Function01:56

Structural Protein Function

30.1K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to...
30.1K
Structural Protein Function01:56

Structural Protein Function

3.3K
3.3K
Mechanical Protein Functions01:58

Mechanical Protein Functions

5.7K
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force. 
5.7K
Protein-protein Interfaces02:04

Protein-protein Interfaces

14.8K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.8K
Activation and Inactivation of G Proteins01:22

Activation and Inactivation of G Proteins

11.7K
Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...
11.7K
Mechanical Protein Function01:58

Mechanical Protein Function

2.5K
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The C-terminal domain of RD3 enables accumulation of retinal membrane guanylyl cyclase (RetGC) in photoreceptor outer segment.

The Journal of biological chemistry·2026
Same author

Protein Inhibitor of Retinal Membrane Guanylyl Cyclase Rescues Mouse Rod Photoreceptors from <i>GUCY2D</i> Retinal Dystrophy.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
Same author

Structural Insights into L-Type Voltage-Gated Ca<sup>2+</sup> Channel (Ca<sub>V</sub>1.2) Activation by CaBP1.

Biochemistry·2026
Same author

Ultrafast Synthesis of Titanium Suboxide via Magnetic Induction Heating for Enhanced Photodynamic Activity.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Single-Dose Liposomal Amphotericin Plus Fluconazole and Flucytosine for Cryptococcal Meningitis at a US Public Hospital.

JAMA network open·2026
Same author

Protein inhibitor of retinal membrane guanylyl cyclase suppresses cGMP synthesis in photoreceptors.

The Journal of biological chemistry·2025

Related Experiment Video

Updated: Feb 13, 2026

Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

3.7K

Retinal guanylyl cyclase activating protein 1 forms a functional dimer.

Sunghyuk Lim1, Graham Roseman2, Igor Peshenko3

  • 1Department of Chemistry, University of California, Davis, CA, United States of America.

Plos One
|March 8, 2018
PubMed
Summary

The guanylyl cyclase activator protein 1 (GCAP1) forms dimers, crucial for its function in vertebrate photoreceptors. This dimerization impacts GCAP1

More Related Videos

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

8.3K
Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
09:07

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay

Published on: December 19, 2018

6.8K

Related Experiment Videos

Last Updated: Feb 13, 2026

Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

3.7K
Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

8.3K
Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
09:07

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay

Published on: December 19, 2018

6.8K

Area of Science:

  • Biochemistry
  • Structural Biology
  • Molecular Biophysics

Background:

  • Retinal guanylyl cyclases (RetGCs) are key regulators in vertebrate photoreceptors.
  • Guanylyl cyclase activator proteins (GCAP1 and GCAP2) modulate RetGC activity.

Purpose of the Study:

  • To determine the atomic resolution structure of the GCAP1 dimer.
  • To elucidate the role of GCAP1 dimerization in photoreceptor function.

Main Methods:

  • Electron Paramagnetic Resonance (EPR) double electron-electron resonance (DEER) spectroscopy.
  • Site-directed mutagenesis and molecular docking.

Main Results:

  • A structural model of the GCAP1 dimer was determined using DEER restraints.
  • The GCAP1 dimer interface involves hydrophobic contacts.
  • Mutations at the interface disrupted dimerization and abolished GCAP1 cyclase-activating function.

Conclusions:

  • GCAP1 dimerization is essential for its regulatory activity.
  • Dimerization may influence GCAP1 compartmentalization and cyclase regulation in photoreceptors.