Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

pre-mRNA Processing02:01

pre-mRNA Processing

57.6K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
57.6K
RNA Splicing01:32

RNA Splicing

60.7K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.7K
Alternative RNA Splicing02:18

Alternative RNA Splicing

25.2K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
25.2K
Ribozymes02:47

Ribozymes

13.5K
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...
13.5K
Organization of Genes02:07

Organization of Genes

73.7K
Overview
73.7K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

11.9K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
11.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comprehensive mapping of the 5' and 3' untranslated regions of <i>Aspergillus fumigatus</i> reveals diverse mechanisms of mRNA processing including premature transcription termination.

RNA (New York, N.Y.)·2025
Same author

Celebrating the fifth edition of the International Symposium on Fungal Stress - ISFUS, a decade after its 2014 debut.

Fungal biology·2025
Same author

Characterizing extracellular vesicles of human fungal pathogens.

Nature microbiology·2025
Same author

<i>Cryptococcus neoformans</i> infections: aspartyl protease potential to improve outcome in susceptible hosts.

mBio·2024
Same author

Alternative TSS use is widespread in Cryptococcus fungi in response to environmental cues and regulated genome-wide by the transcription factor Tur1.

PLoS biology·2024
Same author

The spliceosome impacts morphogenesis in the human fungal pathogen <i>Candida albicans</i>.

mBio·2024

Related Experiment Video

Updated: Feb 10, 2026

Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans
08:24

Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans

Published on: February 27, 2018

14.4K

Introns in Cryptococcus.

Guilhem Janbon1

  • 1Unité Biologie des ARN des Pathogènes Fongiques, Département de Mycologie, Institut Pasteur, Paris, France.

Memorias Do Instituto Oswaldo Cruz
|March 8, 2018
PubMed
Summary

Cryptococcus neoformans introns are essential for gene expression and genome stability. These fungal introns play key roles in regulating gene activity and preventing harmful genetic element spread.

Area of Science:

  • Molecular Biology
  • Mycology
  • Genetics

Background:

  • Cryptococcus neoformans, a pathogenic yeast, possesses introns in nearly all its genes.
  • Recent advances in genome annotation and genetic analysis have highlighted the significant biological functions of these introns.

Purpose of the Study:

  • To elucidate the multifaceted roles of introns in the biology of Cryptococcus neoformans.
  • To establish C. neoformans as a model organism for studying fungal introns.

Main Methods:

  • Genome annotation and genetic analysis were employed.
  • Studies focused on gene expression, alternative splicing, and genome integrity.

Main Results:

  • Introns are crucial for gene expression in C. neoformans.

More Related Videos

Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans
11:07

Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans

Published on: December 19, 2014

13.1K
Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy
10:02

Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy

Published on: October 21, 2014

12.2K

Related Experiment Videos

Last Updated: Feb 10, 2026

Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans
08:24

Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans

Published on: February 27, 2018

14.4K
Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans
11:07

Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans

Published on: December 19, 2014

13.1K
Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy
10:02

Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy

Published on: October 21, 2014

12.2K
  • Alternative splicing of introns regulates gene expression in response to environmental stimuli.
  • Introns contribute to preventing transposon dissemination and maintaining genome integrity.

    • Conclusions:

    • Cryptococcal introns are vital for fundamental cellular processes, including gene expression and genome defense.
    • The unique characteristics of C. neoformans introns suggest its utility as a model system for broader fungal intron research.