Related Experiment Video
Updated: Feb 13, 2026
08:07
Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
Published on: August 2, 2015
8.5K
Comparative Protein Interaction Network Analysis Identifies Shared and Distinct Functions for the Human ROCO Proteins
James E Tomkins1, Sybille Dihanich2, Alexandra Beilina3
1School of Pharmacy, University of Reading, Whiteknights Campus, Reading, UK.
Proteomics
|March 8, 2018
Summary
Researchers mapped human ROCO protein interactions using literature and microarray data. They identified shared functions like stress response and cell projection organization, revealing specialized roles for different ROCO proteins in cellular signaling.
Area of Science:
- Cellular Biology
- Molecular Signaling
- Proteomics
Background:
- Signal transduction relies on kinases and GTPases, with protein-protein interactions (PPIs) influencing cellular processes.
- Human ROCO proteins possess dual kinase/GTPase activities and scaffolding functions, making them ideal models for studying PPI signaling.
Purpose of the Study:
- To investigate PPI signaling events involving human ROCO proteins.
- To construct comprehensive PPI networks for ROCO proteins using complementary methods.
- To identify conserved and unique functions within the ROCO protein family.
Main Methods:
- Weighted PPI network analysis (WPPINA) pipeline to extract validated ROCO interactors from literature.
- Experimental assessment of novel ROCO PPIs using protein microarray screens.
- Comparative analysis and functional enrichment of networks derived from both approaches.
Main Results:
- Construction of confidence-weighted PPI networks for human ROCO proteins.
- Identification of common interactors and shared functions, including stress response and cell projection organization.
- Evidence for specialized cellular roles among different ROCO protein family members due to unique interactors.
Conclusions:
- Integrated PPI data from literature and experimental screens provides high-resolution insights into ROCO protein interactions.
- ROCO proteins share core functions but also exhibit specialized roles, highlighting the complexity of cellular signaling.
- This multi-approach strategy serves as a model for leveraging PPI data integration in signaling biology research.
Related Concept Videos
02:26Protein Networks
4.6K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.6K
02:26Protein Networks
2.9K
2.9K
01:56Structural Protein Function
30.1K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
30.1K
01:56Structural Protein Function
3.3K
3.3K
01:58Mechanical Protein Functions
5.7K
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force.
5.7K
02:26Conservation of Protein Domains Over Different Proteins
14.7K
Protein domains are small structurally independent units that are part of a single amino acid chain. Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
14.7K