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Calcium fluoride containing granules produced in vitro in rat bones.
Scandinavian Journal of Dental Research
|October 1, 1979
Summary
Fluoride fixation in rat bones revealed calcium fluoride (CaF2) granules in various bone and cartilage tissues. These granules, identified via microscopy and diffraction, may represent fixation artifacts.
Area of Science:
- Biomaterials Science
- Skeletal Biology
- Histology
Background:
- Sodium fluoride (NaF) fixation is used in biological sample preparation.
- Demineralization processes can potentially alter tissue composition.
- Understanding artifacts is crucial for accurate histological interpretation.
Purpose of the Study:
- To investigate the nature of fluoride-containing granules observed in NaF-fixed rat bones.
- To determine if these granules represent genuine tissue components or fixation artifacts.
Main Methods:
- Rat femurs and tibias were fixed using glutaraldehyde or formaldehyde with NaF.
- Radiographically controlled demineralization was performed using nitric acid (HNO3).
- Light and transmission electron microscopy, selected area diffraction, and X-ray diffraction were employed.
Main Results:
- Fluoride granules were identified in bone trabeculae, cartilage, and bone marrow.
- Selected area diffraction confirmed the presence of calcium fluoride (CaF2) within these granules.
- X-ray diffraction of non-demineralized samples did not detect CaF2.
Conclusions:
- The observed CaF2 granules are likely precipitation artifacts formed during the demineralization of NaF-fixed tissues.
- These findings are relevant to the interpretation of similar granules in other studies of demineralized hard tissues.